Phylogenetic analysis based on mitochondrial DNA sequences of wild rats, and the relationship with Seoul virus infection in Hubei, China
Phylogenetic analysis based on mitochondrial DNA sequences of wild rats, and the relationship with Seoul virus infection in Hubei, China
复制标题
基于野生大鼠线粒体DNA序列的系统发育分析及其与中国湖北首尔病毒感染的关系。
DOI：
10.1007/s12250-016-3940-0
复制
发表时间：
2017-06-01
影响因子：
5.5
通讯作者：
Yang, Zhan-Qiu
中科院分区：
文献类型：
--
作者：
Liu, Dong-Ying;Liu, Jing;Yang, Zhan-Qiu
首尔病毒是中国地区肾综合征出血热的主要病原之一，主要由褐家鼠携带。湖北省位于中国的中南部，经历了一些最严重的肾综合征出血热疫情。为探讨湖北野鼠线粒体DNA(MtDNA)系统发育及其与SEOV感染的关系，从2000-2009年和2014-2015年在湖北5个捕鼠点捕获了664只野鼠。逆转录-聚合酶链式反应(RT-PCR)发现41只(6.17%)大鼠SEOV感染阳性。宜昌市SEOV阳性率明显低于其他地区。对103只大鼠的线粒体DNA D-loop和细胞色素b(Cyt-b)基因进行了测序。在这些动物中，有37只是SEOV阳性。系统发育关系的重建(基于完整的D-环和Cyt-b序列)允许将大鼠分为两个谱系，即褐家鼠和两栖家鼠，前者包括大多数大鼠。D-loop和Cyt-b基因均鉴定出18种单倍型。不同单倍型的地理分布有显著差异。不同单倍型间SEOV阳性百分率差异无统计学意义。D-loop有三个亚系，cyt-b有两个亚系。各亚系的SEOV阳性百分率没有显著差异。提示SEOV阳性百分率与湖北大鼠mtDNA D-loop或细胞单倍型或亚系无关。
Seoul virus (SEOV), which is predominantly carried byRattus norvegicus, is one of the major causes of hemorrhagic fever with renal syndrome (HFRS) in China. Hubei province, located in the central south of China, has experienced some of the most severe epidemics of HFRS. To investigate the mitochondrial DNA (mtDNA)-based phylogenetics of wild rats in Hubei, and the relationship with SEOV infection, 664 wild rats were captured from five trapping sites in Hubei from 2000–2009 and 2014–2015. Using reverse-transcription (RT)-PCR, 41 (6.17%) rats were found to be positive for SEOV infection. The SEOV-positive percentage in Yichang was significantly lower than that in other areas. The mtDNA D-loop and cytochrome b (cyt-b) genes of 103 rats were sequenced. Among these animals, 37 were SEOV-positive. The reconstruction of the phylogenetic relationship (based on the complete D-loop andcyt-bsequences) allowed the rats to be categorized into two lineages,R. norvegicusandRattus nitidus, with the former including the majority of the rats. For both the D-loop andcyt-bgenes, 18 haplotypes were identified. The geographic distributions of the different haplotypes were significantly different. There were no significant differences in the SEOVpositive percentages between different haplotypes. There were three sub-lineages for the D-loop, and two forcyt-b. The SEOV-positive percentages for each of the sub-lineages did not significantly differ. This indicates that the SEOV-positive percentage is not related to the mtDNA D-loop orcyt-bhaplotype or the sub-lineage of rats from Hubei.