Dipolar Order Parameters in Large Systems With Fast Spinning.
Dipolar Order Parameters in Large Systems With Fast Spinning.
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DOI：
10.3389/fmolb.2021.791026
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发表时间：
2021
影响因子：
5
通讯作者：
Lewandowski JR
中科院分区：
文献类型：
--
作者：
Franks WT;Tatman BP;Trenouth J;Lewandowski JR
序参数是量化分子运动幅度的有用工具。在这里，我们测量偶极序参数的重耦合下的快速旋转的异质偶极偶极耦合。我们采用基于对称性的再耦合方法，在60 kHz的魔角下旋转的样品，采用可变翻转角复合反转脉冲。我们验证的方法，通过测量特定位点的15 N-1H顺序参数的微晶蛋白在一个小的温度范围内，同一蛋白质在一个大的，沉淀的复合物与抗体。在复杂的顺序参数的测量是一致的，所观察到的蛋白质进行整体运动内组装。
Order parameters are a useful tool for quantifying amplitudes of molecular motions. Here we measure dipolar order parameters by recoupling heteronuclear dipole-dipole couplings under fast spinning. We apply symmetry based recoupling methods to samples spinning under magic angle at 60 kHz by employing a variable flip angle compound inversion pulse. We validate the methods by measuring site-specific 15N-1H order parameters of a microcrystalline protein over a small temperature range and the same protein in a large, precipitated complex with antibody. The measurements of the order parameters in the complex are consistent with the observed protein undergoing overall motion within the assembly.