Simultaneous quantitation of 14 active components in Yinchenhao decoction by using ultra high performance liquid chromatography with diode array detection: Method development and ingredient analysis of different commonly prepared samples.
Simultaneous quantitation of 14 active components in Yinchenhao decoction by using ultra high performance liquid chromatography with diode array detection: Method development and ingredient analysis of different commonly prepared samples.
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DOI：
10.1002/jssc.201600284
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发表时间：
2016-09
影响因子：
3.1
通讯作者：
Yaxiong Yi;Yong Zhang;Yue Ding;Lu Lu-Lu;Tong Zhang;Yuan Zhao;Xiaojun Xu;Yuxin Zhang
中科院分区：
文献类型：
--
作者：
Yaxiong Yi;Yong Zhang;Yue Ding;Lu Lu-Lu;Tong Zhang;Yuan Zhao;Xiaojun Xu;Yuxin Zhang
建立了一种基于超高效液相色谱-二极管阵列检测器（UPLC-DAD）同时测定茵陈蒿汤中14种主要活性成分的新方法。采用Agilent SB-C18色谱柱，乙腈/0.1%磷酸溶液梯度洗脱，流速为0.4 mL/min，洗脱时间为35 min，进行线性、精密度、重复性和准确度试验。应用该方法对8个茵陈蒿汤常规提取样品中的14个组分进行了成分差异分析，并进行了系统聚类分析和相似性分析。结果表明，水煎法、水煎法、超声法、水提取法和乙醇提取法均影响中药饮片的成分鉴别，应与其临床应用相结合。结果还表明，由家庭中的患者使用砂锅水提取制备的样品与使用制药厂中主要使用的不锈钢釜制备的样品几乎相同。本研究有助于临床选择最佳、最方便的茵陈蒿汤的制备方法。
We developed a novel quantitative analysis method based on ultra high performance liquid chromatography coupled with diode array detection for the simultaneous determination of the 14 main active components in Yinchenhao decoction. All components were separated on an Agilent SB-C18 column by using a gradient solvent system of acetonitrile/0.1% phosphoric acid solution at a flow rate of 0.4 mL/min for 35 min. Subsequently, linearity, precision, repeatability, and accuracy tests were implemented to validate the method. Furthermore, the method has been applied for compositional difference analysis of 14 components in eight normal-extraction Yinchenhao decoction samples, accompanied by hierarchical clustering analysis and similarity analysis. The result that all samples were divided into three groups based on different contents of components demonstrated that extraction methods of decocting, refluxing, ultrasonication and extraction solvents of water or ethanol affected component differentiation, and should be related to its clinical applications. The results also indicated that the sample prepared by patients in the family by using water extraction employing a casserole was almost same to that prepared using a stainless-steel kettle, which is mostly used in pharmaceutical factories. This research would help patients to select the best and most convenient method for preparing Yinchenhao decoction.