Proteomics and the inner ear.
Proteomics and the inner ear.
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蛋白质组学和内耳。
DOI：
10.1155/2001/476738
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
Thalmann,I
中科院分区：
文献类型：
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作者：
Thalmann,I
内耳是最复杂的器官之一，在其骨壳中包含三个感觉系统，进化最古老的重力受体系统，用于检测角加速度的三个半圆形管道，以及听觉系统-在灵敏度和频率识别方面无与伦比。这三个系统都容易受到一系列影响我们所有人生活质量的困扰。在这篇综述的第一部分，我们介绍了内耳研究的里程碑，为理解蛋白质组学方法的复杂性铺平了道路。微小的感觉结构，被巨大的流体空间和坚硬的骨壳包围，给耳朵研究人员带来了极大的挑战。尽管存在这些障碍，但一种强大的准备技术被开发出来，通过这种技术，精确定义的微观组织元素可以被分离和分析，同时保持代表其体内条件的生化状态。第二部分包括讨论蛋白质组学作为一种工具在阐明基本和病理机制，疾病的诊断，以及治疗。例如Corti蛋白OCP1和OCP2的器官、肿瘤调节蛋白（一种高度特异性的钙结合蛋白）和一些疾病实体，如梅尼埃病、良性阵发性位置性眩晕和淋巴管周围瘘管。
The inner ear, one of the most complex organs, contains within its bony shell three sensory systems, the evolutionary oldest gravity receptor system, the three semicircular canals for the detection of angular acceleration, and the auditory system ‐ unrivaled in sensitivity and frequency discrimination. All three systems are susceptible to a host of afflictions affecting the quality of life for all of us. In the first part of this review we present an introduction to the milestones of inner ear research to pave the way for understanding the complexities of a proteomics approach to the ear. Minute sensory structures, surrounded by large fluid spaces and a hard bony shell, pose extreme challenges to the ear researcher. In spite of these obstacles, a powerful preparatory technique was developed, whereby precisely defined microscopic tissue elements can be isolated and analyzed, while maintaining the biochemical state representative of thein vivoconditions. The second part consists of a discussion of proteomics as a tool in the elucidation of basic and pathologic mechanisms, diagnosis of disease, as well as treatment. Examples are the organ of Corti proteins OCP1 and OCP2, oncomodulin, a highly specific calcium‐binding protein, and several disease entities, Meniere′s disease, benign paroxysmal positional vertigo, and perilymphatic fistula.