A Drosophila salivary gland mucin is also expressed in immune tissues:: evidence for a function in coagulation and the entrapment of bacteria
A Drosophila salivary gland mucin is also expressed in immune tissues:: evidence for a function in coagulation and the entrapment of bacteria
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DOI：
10.1016/j.ibmb.2004.09.001
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发表时间：
2004-12-01
影响因子：
3.8
通讯作者：
Theopold, U
中科院分区：
文献类型：
--
作者：
Korayem, AM;Fabbri, M;Theopold, U
我们对果蝇糖基化的发育调控的研究使我们确定和表征gp 150，一种蜕皮激素调节的粘蛋白，发现于血细胞、肠（围食膜）和唾液腺中。我们对粘蛋白免疫功能特别感兴趣，发现gp 150从幼虫血细胞中释放出来，成为凝块的一部分，并参与细菌的捕获。通过RT-PCR和RNAi实验，我们鉴定了gp 150为先前描述的蜕皮激素诱导的唾液胶蛋白171-7。我们讨论了进化和生物化学的影响，在昆虫的免疫功能的唾液蛋白的双重用途。对这些共有蛋白质的进一步分子表征可以使人们更好地理解参与微生物的遏制和消除以及止血和伤口修复的蛋白质的性质。(C)2004爱思唯尔有限公司保留所有权利。
Our studies on the developmental regulation of glycosylation in Drosophila melanogaster led us to identify and characterize gp 150, an ecdysone-regulated mucin that is found in hemocytes, the gut (peritrophic membrane) and in the salivary glands. We are particularly interested in mucin immune functions and found that gp 150 is released from larval hemocytes, becomes part of the clot and participates in the entrapment of bacteria. By RT-PCR and RNAi experiments, we identified gp 150 as the previously described 171-7, an ecdysone-induced salivary glue protein. We discuss the evolutionary and biochemical implications of the dual use of salivary proteins for immune functions in insects. Further molecular characterization of such shared proteins may enable a better understanding of the properties of proteins involved in containment and elimination of microbes, as well as hemostasis and wound repair. (C) 2004 Elsevier Ltd. All rights reserved.