Bone-marrow derived hematopoietic stem/progenitor cells express multiple isoforms of NADPH oxidase and produce constitutively reactive oxygen species
Bone-marrow derived hematopoietic stem/progenitor cells express multiple isoforms of NADPH oxidase and produce constitutively reactive oxygen species
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DOI：
10.1016/j.bbrc.2006.12.148
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发表时间：
2007-02-23
影响因子：
3.1
通讯作者：
Capitanio, Nazzareno
中科院分区：
文献类型：
--
作者：
Piccoli, Claudia;D'Aprile, Annamaria;Capitanio, Nazzareno
综合证据强调了氧化还原信号在平衡成体干细胞自我更新和分化之间的平衡中的重要性。本研究表明，人hernatopoictic干/祖细胞（HSC）组成性产生低水平的过氧化氢，其生产被DPI，夹竹桃苷，过氧化氢酶，和LY 294002抑制，几乎没有刺激PMA。此外，显示HSC在mRNA和蛋白质水平表达NADPH氧化酶家族的NOX 1、NOX 2和NOX 4同种型的催化亚基沿着调节亚基p22、p40、p47、p67、rac 1、rac 2、NOXI 1和NOXA 1以及剪接变体NOX 2s的完整电池，并且三种NOX同种型在同一HSC中大部分共表达。这些发现被解释为NOX激活的正反馈机制，使得ROS水平的微调可能用于HSC生长和分化的氧化还原介导的信号传导。(c)2006年爱思唯尔公司All rights reserved.
Consolidated evidence highlights the importance of redox signalling in poising the balance between self-renewal and differentiation in adult stem cells. The present study shows that human hernatopoictic stem/progenitor cells (HSCs) constitutively generate low levels of hydrogen peroxide whose production is inhibited by DPI, apocynin, catalase, and LY294002 and scarcely stimulated by PMA. Moreover, it is shown that HSCs express at the mRNA and protein levels the catalytic subunits of NOX1, NOX2, and NOX4 isoforms of the NADPH oxidase family along with the complete battery of the regulatory subunits p22, p40, p47, p67, rac1, rac2, NOXI1, and NOXA1 as well as the splicing variant NOX2s and that the three NOX isoforms are largely co-expressed in the same HSC. These findings are interpreted in terms of a positive feed-back mechanism of NOXs activation enabling a fine tuning of the ROS level to be possibly used in redox-mediated signalling for growth and differentiation of HSCs. (c) 2006 Elsevier Inc. All rights reserved.