Mouse mutagenesis with the chemical supermutagen ENU.
Mouse mutagenesis with the chemical supermutagen ENU.
复制标题
小鼠诱变与化学超木素ENU。
DOI：
10.1016/s0076-6879(10)77015-4
复制
发表时间：
2010
影响因子：
--
通讯作者：
Justice MJ
中科院分区：
文献类型：
--
作者：
Probst FJ;Justice MJ
小鼠生殖系突变的产生和分析是现代生物学研究的基石之一。化学超级诱变剂N-乙基-N-亚硝基脲（ENU）是已知最有效的小鼠诱变剂，可用于在整个小鼠基因组中产生点突变。ENU诱变的雄性的后代可以筛选常染色体显性表型，或者它们可以用于产生多代谱系以筛选常染色体隐性性状。将平衡染色体引入育种方案可以允许选择性捕获特定染色体区域中的突变。最近的工作已经证明，使用已经具有感兴趣的突变的动物可以导致成功分离修饰原始突变表型的额外突变。此外，现代分子技术确保突变可以容易地被识别。我们在这里描述了用ENU诱变雄性小鼠的程序，并解释了可以对不同类型的诱导突变进行的各种类型的筛选。目前发表的关于小鼠ENU诱变的研究仅仅触及了这种强大技术的表面，进一步的工作肯定会加深我们对小鼠基因组各个组成部分的作用以及它们之间无数关系的认识。
The generation and analysis of germline mutations in the mouse is one of the cornerstones of modern biological research. The chemical supermutagen N-ethyl-N-nitrosourea (ENU) is the most potent known mouse mutagen and can be used to generate point mutations throughout the mouse genome. The progeny of ENU-mutagenized males can be screened for autosomal dominant phenotypes, or they can be used to generate multigeneration pedigrees to screen for autosomal recessive traits. The introduction of balancer chromosomes into the breeding scheme can allow for the selective capture of mutations in a specific chromosomal region. More recent work has demonstrated that the use of animals that already have a mutation of interest can lead to the successful isolation of additional mutations that modify the original mutant phenotype. Further, modern molecular techniques ensure that mutations can be readily identified. We describe here the procedures for mutagenizing male mice with ENU and explain the various types of screens that can be performed for different kinds of induced mutations. The currently published research on ENU mutagenesis in the mouse has only scratched the surface of what is possible with this powerful technique, and further work is certain to deepen our knowledge of the role of the individual components of the mouse genome and the myriad relationships between them.