A reversible and highly selective fluorescent sensor for mercury(II) using poly (thiophene)s that contain thymine moieties
A reversible and highly selective fluorescent sensor for mercury(II) using poly (thiophene)s that contain thymine moieties
复制标题
使用含有胸腺嘧啶部分的聚噻吩的可逆、高选择性汞 (II) 荧光传感器
DOI：
10.1002/marc.200500837
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发表时间：
2006-03-24
影响因子：
4.6
通讯作者：
Zhu, DB
中科院分区：
文献类型：
--
作者：
Tang, YL;He, F;Zhu, DB
建立了一种用共轭聚合物(CP)测定汞(II)(Hg2+)离子的可逆、高选择性分析方法。其转导机制是基于Hg2+介导的聚合物间pi堆积聚集，导致CP的荧光自猝灭。合成了含胸腺嘧啶基团的CP-poly[3-(N-thymin-1-ylacetyl)ethylamine-thiophene](PTT)，并对其进行了表征。在没有Hg2+离子的情况下，PTT链保持相互分离，而CP显示出很强的荧光发射。加入Hg2+离子后，由于胸腺嘧啶-汞-胸腺嘧啶的特异性相互作用，形成了聚合物间的聚集态，导致了PTT的荧光猝灭。该方法的特点包括CPS的信号放大和Hg2+离子与胸腺嘧啶-胸腺嘧啶(T-T)碱基对的特异性结合。
A reversible and highly selective assay method has been developed to detect mercury(II) (Hg2+) ions using a conjugated polymer (CP). The transduction mechanism is based on Hg2+-mediated interpolymer pi-stacking aggregation, which results in the fluorescence self-quenching of the CP. CPs that contain thymine moieties, poly[3-(N-thymin-1-ylacetyl)ethylamine-thiophene] (PTT), have been synthesized and characteized. In the absence of Hg2+ ions, the PTT chains remain separated from each other and the CP exhibits strong fluorescence emission. Upon adding Hg2+ ions, the formation of interpolymer pi-stacking aggregation induced by specific thymine-Hg-thymine interactions results in the fluorescence quenching of PTT. Distinguishing aspects of this assay include the signal amplification of CPs and the specific binding of Hg2+ ions to thymine-thymine (T-T) base pairs.