Modification of chemotherapeutic effects on L1210 cells using hematoporphyrin and light.
Modification of chemotherapeutic effects on L1210 cells using hematoporphyrin and light.
复制标题
使用血卟啉和光改变 L1210 细胞的化疗效果。
作者：
S. Creekmore;D. Zaharko
利用光和血卟啉（HP）对L1210细胞产生中等毒性的光动力学损伤，通过组织培养实验研究了苯丙氨酸氮芥（LPAM）和放线菌素D对L1210细胞的光动力学反应曲线的改变。细胞在含有20%马血清的Roswell Park Memorial Institute Medium 1630中生长和处理。将细胞与浓度为1至50 μ M的HP孵育长达48小时。通过荧光（4毫瓦/平方厘米）提供照明，通过塑料过滤以去除400至800 nm范围外的所有波长。环境光线被仔细控制。当细胞与25 μ M HP在黑暗中孵育24小时，然后暴露于光1小时时，与黑暗HP处理的对照相比，克隆效率降低了30%至80%。当LPAM（1至30 μ M）或放线菌素D（0.04至2.0ng/ml）在HP处理后与细胞一起孵育1小时时，在有或没有光暴露的情况下，LPAM响应曲线被修改以指示光动力毒性和化疗毒性的协同响应（一个额外的log）。在LPAM暴露之前和期间在黑暗中的HP没有修改LPAM响应曲线。放线菌素D响应曲线通过先前的HP和光处理进行修改，以表明一个额外的log的累加效应;在100至10%的克隆效率范围内可能存在协同效应。可以得出结论，L1210-HP-光系统提供了可能性，调查化疗效果的修改。
Tissue culture experiments were done to evaluate the possibility of modifying the response curves of phenylalanine mustard (LPAM) and actinomycin D on L1210 cells, using moderately toxic levels of photodynamic injury provided by light and hematoporphyrin (HP). Cells were grown and treated in Roswell Park Memorial Institute Medium 1630 containing 20% horse serum. Cells were incubated with HP at concentrations of 1 to 50 microM for up to 48 hr. Illumination was provided by fluorescent light (4 milliwatts/sq cm), filtered through plastic to remove all wavelengths outside of the range of 400 to 800 nm. Ambient light was carefully controlled. When cells were incubated with 25 microM HP in the dark for 24 hr and then exposed to light for 1 hr, there were reductions in cloning efficiencies of 30 to 80% compared to the dark-HP-treated controls. When LPAM (1 to 30 microM) or actinomycin D (0.04 to 2.0 ng/ml) was incubated with cells for 1 hr following HP treatment, with or without light exposure, the LPAM response curves were modified to indicate a synergistic response of photodynamic toxicity and chemotherapeutic toxicity (one additional log). HP in the dark prior to and during LPAM exposure did not modify the LPAM response curve. The actinomycin D response curve was modified by prior HP and light treatment to indicate an additive effect of one additional log; a synergistic effect may be present in the range of 100 to 10% cloning efficiencies. It is concluded that the L1210-HP-light system offers possibilities for investigating the modification of chemotherapy effects.