Effects of endothelium-derived relaxing factor and nitric oxide on rat mesangial cells.
Effects of endothelium-derived relaxing factor and nitric oxide on rat mesangial cells.
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内皮源性舒张因子和一氧化氮对大鼠系膜细胞的影响。
DOI：
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发表时间：
1990
影响因子：
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通讯作者：
Leopoldo Raij
中科院分区：
文献类型：
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作者：
Pamela J. Shultz;A. Schorer;Leopoldo Raij
我们研究了内皮源性舒张因子（EDRF）和一氧化氮（NO），一种被认为是EDRF之一的物质，是否可以引起大鼠肾小球系膜细胞（MC）的生化和生物学反应。在仅含有MC的威尔斯孔中，鸟苷3 '，5'-环一磷酸（cGMP）水平为2.6 +/- 0.6 fmol/微克蛋白质，缓激肽不影响这些水平，而在牛主动脉EC和大鼠MC的共孵育实验中，MC中的cGMP水平增加至44.6 +/- 21 fmol/微克蛋白质缓激肽刺激后（P小于0.05）。超氧化物歧化酶可增强这种作用，血红蛋白和EDRF合成的特异性抑制剂L-NG-单甲基精氨酸可抑制这种作用。当MC直接与NO孵育时也观察到cGMP的增加，并且被超氧化物歧化酶增强，被血红蛋白抑制。我们还测试了NO是否可以抑制血管紧张素II（ANG II）诱导的MC横截面积（CSA）的减少。当MC仅暴露于ANG II时，通过数字图像分析测量，65%的细胞经历了CSA的显著减少。而当MC与ANG Ⅱ和NO共同孵育时，只有10%的细胞有反应（P <0.04）。这些研究表明，EDRF和NO诱导大鼠肾小球MC显着的生化和功能反应，并建议EC和MC之间的通信可能是重要的肾小球功能的调节。
We have investigated whether endothelium-derived relaxing factor (EDRF) and nitric oxide (NO), a substance proposed to be one of the EDRFs, could elicit biochemical and biological responses in rat glomerular mesangial cells (MC). In wells with MC alone, guanosine 3',5'-cyclic monophosphate (cGMP) levels were 2.6 +/- 0.6 fmol/microgram protein, and bradykinin did not affect these levels, whereas in coincubation experiments with bovine aortic EC and rat MC, cGMP levels in MC increased to 44.6 +/- 21 fmol/micrograms protein after bradykinin stimulation (P less than 0.05). This effect was potentiated by superoxide dismutase and inhibited by hemoglobin and L-NG-monomethyl arginine, a specific inhibitor of EDRF synthesis. Increases in cGMP were also observed when MC were incubated directly with NO and were potentiated by superoxide dismutase and inhibited by hemoglobin. We also tested whether NO could inhibit angiotensin II (ANG II)-induced reductions in cross-sectional area (CSA) of MC. When MC were exposed to ANG II only, 65% of the cells underwent a significant reduction in CSA, as measured by digital image analysis. However, when MC were incubated with ANG II and NO, only 10% of cells responded (P less than 0.04). These studies demonstrate that EDRF and NO induce significant biochemical and functional responses in rat glomerular MC and suggest that communication between EC and MC may be important in regulation of glomerular function.