Biofunctionalized PEDOT-coated microfibers for the treatment of spinal cord injury
Biofunctionalized PEDOT-coated microfibers for the treatment of spinal cord injury
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DOI：
10.1016/j.biomaterials.2016.02.037
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发表时间：
2016-05-01
期刊：
影响因子：
14
通讯作者：
Collazos-Castro, Jorge E.
中科院分区：
文献类型：
--
作者：
Alves-Sampaio, Alexandra;Garcia-Rama, Concepcion;Collazos-Castro, Jorge E.
聚（3，4-乙撑二氧噻吩）涂层碳微纤维（PEDOT-MFs）有望开发先进的神经假体和神经修复装置。我们研究了慢性细胞反应PEDOT-MFs植入未受伤和横断大鼠脊髓，并比较了聚合物表面生物功能化与共价连接的聚赖氨酸（PLL）或PLL，肝素，碱性成纤维细胞生长因子（bFGF），和纤连蛋白的多分子复合物的影响。藻酸盐凝胶用于促进微纤维植入并减少脊髓损伤（SCI）后的结缔组织瘢痕形成。PLL/肝素/bFGF/纤连蛋白功能化的PEDOT-MF在未损伤和损伤的脊髓内显示出优异的整合，经常与神经元胞体、轴突、树突和神经胶质细胞建立接触，伴随着非常少或不存在瘢痕形成反应。相反，非功能化和PLL功能化的微纤维引起炎症和纤维化，周围组织中的神经元素损失。在病变内，PEDOT-MF本身促进迁移细胞和生长轴突的纵向排列，并且它们用PLL/肝素/bFGF/纤连蛋白修饰促进组织愈合，增强血管形成和轴突再生而不增加炎症。这些结果支持将生物功能化的导电微纤维纳入神经电子接口和损伤桥接系统用于治疗SCI。(C)2016爱思唯尔有限公司版权所有
PoIy(3, 4-ethylenedioxythiophene)-coated carbon microfibers (PEDOT-MFs) hold promise for developing advanced neuroprostheses and neural repair devices. We investigated the chronic cellular responses to PEDOT-MFs implanted into the uninjured and the transected rat spinal cord, and compared the effects of polymer surface biofunctionalization with covalently attached polylysine (PLL) or a multimolecular complex of PLL, heparin, basic fibroblast growth factor (bFGF), and fibronectin. An alginate gel was used to facilitate microfiber implantation and reduce connective tissue scarring after spinal cord injury (SCI). PLL/heparin/bFGF/fibronectin-functionalized PEDOT-MFs showed excellent integration within the uninjured and injured spinal cord, frequently establishing contact with neuronal somas, axons, dendrites and glial cells, accompanied by very little or absent scarring response. On the contrary, non-functionalized and PLL-functionalized microfibers provoked inflammation and fibrosis with loss of neural elements in the surrounding tissue. Within the lesion, the PEDOT-MFs by themselves facilitated longitudinal alignment of migratory cells and growing axons, and their modification with PLL/heparin/bFGF/fibronectin promoted tissue healing, enhancing blood vessel formation and axonal regeneration without increasing inflammation. These results support the incorporation of biofunctionalized electro-conducting microfibers in neuro electronic interfaces and lesion-bridging systems for the treatment of SCI. (C) 2016 Elsevier Ltd. All rights reserved.