ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS
ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS
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DOI：
10.1016/0003-2697(82)90118-x
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发表时间：
1982-01-01
影响因子：
2.9
通讯作者：
TANNENBAUM, SR
中科院分区：
文献类型：
--
作者：
GREEN, LC;WAGNER, DA;TANNENBAUM, SR
本文介绍了一种新的高压镉柱还原硝酸盐自动分析系统。尿液、唾液、去蛋白血浆、胃液和牛奶样品可用于分析硝酸盐、亚硝酸盐或两者，检测下限为1.0 nmol NO3-或NO2-/ml。该系统可以定量减少硝酸盐，并自动消除尿液和其他生物液体中通常存在的其他化合物的干扰。分析速度为30个样品/h，大多数样品的制备仅限于用蒸馏水简单稀释。本文还介绍了15NO_2-苯衍生化后的气相色谱/质谱联用技术在尿中15NO_3-分析中的应用。
A new automated system for the analysis of nitrate via reduction with a high-pressure Cd column is described. Samples of urine, saliva, deproteinized plasma, gastric juice and milk can be analyzed for nitrate, nitrite, or both with a lower limit of detection of 1.0 nmol NO3- or NO2-/ml. The system allows quantitative reduction of nitrate and automatically eliminates interference from other compounds normally present in urine and other biological fluids. Analysis rate is 30 samples/h, with preparation for most samples limited to simple dilution with distilled water. The application of gas chromatography/mass spectrometry for the analysis of 15NO3- in urine after derivatization to 15NO2-benzene is also described.