N-glycans show distinct spatial distribution in mouse brain.
N-glycans show distinct spatial distribution in mouse brain.
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N-聚糖在小鼠大脑中表现出明显的空间分布。
DOI：
10.1101/2023.05.30.542954
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Mealer,RobertG
中科院分区：
文献类型：
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作者：
Noel,Maxence;Cummings,RichardD;Mealer,RobertG
大脑的发育和功能需要蛋白质的n -链糖基化，这是分泌途径中普遍存在的修饰。n -聚糖具有独特的组成，并在大脑中受到严格的调节，但这些结构的空间分布仍相对未被探索。在这里，我们系统地使用碳水化合物结合凝集素对不同种类的n -聚糖和适当的对照具有不同的特异性，以鉴定多糖在小鼠大脑多个区域的表达。结合高甘露糖型n -聚糖的凝集素在高倍镜下呈弥漫性染色，可见一些点状结构。结合复杂n -聚糖特异性基序的凝集素，包括聚焦和分割GlcNAc，显示出更多的分区标记，包括到小脑的富含突触的分子层。了解n -聚糖在大脑中的空间分布将有助于未来研究这些关键的蛋白质修饰在大脑发育和疾病中的作用。
The development and function of the brain requires N-linked glycosylation of proteins, which is a ubiquitous modification in the secretory pathway. N-glycans have a distinct composition and undergo tight regulation in the brain, but the spatial distribution of these structures remains relatively unexplored. Here, we systematically employed carbohydrate binding lectins with differing specificities to various classes of N-glycans and appropriate controls to identify glycan expression in multiple regions of the mouse brain. Lectins binding high-mannose-type N-glycans, the most abundant class of brain N-glycans, showed diffuse staining with some punctate structures observed on high magnification. Lectins binding specific motifs of complex N-glycans, including fucose and bisecting GlcNAc, showed more partitioned labeling, including to the synapse-rich molecular layer of the cerebellum. Understanding the spatial distribution of N-glycans across the brain will aid future studies of these critical protein modifications in development and disease of the brain.