Response of in vitro reared honey bee larvae to various doses of Paenibacillus larvae larvae spores
Response of in vitro reared honey bee larvae to various doses of Paenibacillus larvae larvae spores
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DOI：
10.1051/apido:19980609
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发表时间：
1998-11-01
期刊：
影响因子：
2.4
通讯作者：
Hansen, H
中科院分区：
文献类型：
--
作者：
Brodsgaard, CJ;Ritter, W;Hansen, H
分别饲喂24-28、48-52和72-76 h意大利蜜蜂幼虫的类芽孢杆菌幼虫孢子数与P. l.幼虫死亡数之间的关系。研究了幼虫感染以及 P. l 亚致死感染的影响。幼虫对幼虫发育时间和成虫体重的影响。幼虫是在实验室饲养的，因此排除了护士蜂的影响。结果表明，24-28小时龄的幼虫最容易感染P. l.。幼虫具有明确的剂量反应关系（LD50 = 8.49）。年长的幼虫对 P. l. 感染的抵抗力越来越强。幼虫，因此在 48-52 小时和 72-76 小时年龄组中不存在显着的剂量-死亡率关系。此外，未接种组和接种组的成年蜜蜂的发育时间和羽化重量之间均存在显着的负相关。未接种的幼虫和接种后存活的幼虫之间的发育时间没有差异。 (C) Inra/DIB/AGIB/Elsevier，巴黎。
The relationship between the number of Paenibacillus larvae larvae spores fed individually to 24-28, 48-52 and 72-76 h old Apis mellifera ligustica larvae and the number of larvae dying from the P. l. larvae infection was investigated as well as the effect of sublethal infection with P. l. larvae on larval development time and adult weight. The larvae were laboratory reared, thus, excluding the influence of nurse bees. The results show that larvae 24-28 h old are most susceptible to infection with P. l. larvae with a clear dose-response relationship (LD50 = 8.49). Older larvae become more and more resistant to infection with P. l. larvae so that no significant dose-mortality relationship exists in the 48-52 and 72-76 h age groups. Furthermore, a significant negative correlation exists between the development time and emergence weight for the adult bees in both the non-inoculated group and the inoculated group. There was no difference in development time between non-inoculated larvae and those that survived the inoculation. (C) Inra/DIB/AGIB/Elsevier, Paris.