Dynamic changes in blood flow and oxygen consumption in the portal-drained viscera of growing pigs receiving acute administration of L-arginine
Dynamic changes in blood flow and oxygen consumption in the portal-drained viscera of growing pigs receiving acute administration of L-arginine
复制标题
急性施用L-精氨酸的生长猪门静脉引流内脏血流量和耗氧量的动态变化
DOI：
10.1007/s00726-012-1328-5
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发表时间：
2012-12-01
期刊：
影响因子：
3.5
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
--
作者：
Tan, Bie;Li, Xinguo;Yin, Yulong
本研究验证了门静脉精氨酸浓度增加可能影响猪门静脉引流内脏（PDV）血流量和耗氧量的假设。八只公猪（体重70 kg）通过手术在门静脉、回肠静脉和颈动脉中安装慢性导管。手术后13天，禁食12小时的猪被随机分配接受通过门静脉给予l-丙氨酸（103 mg/kg体重，等氮对照）或l-精氨酸-HCl（61 mg/kg体重）。门静脉血流量（PVBF）测定采用对氨基马尿酸（p-aminohypuric acid，PA）回肠静脉滴注法，同时每0.5h采血一次，共4 h。与对照组比较，精氨酸组输注后30-90 min PVBF增加，60-150 min PDV耗氧量降低（P < 0.05）。输注精氨酸180-240 min时血浆谷氨酸浓度和60-240 min时血浆精氨酸浓度均高于对照组（P < 0.05）。输注时间30-90 min时血浆胰岛素和胰高血糖素浓度高于对照组（P <0.05），输注时间60-120 min时血浆游离脂肪酸浓度低于对照组（P < 0.05）。这些结果表明，门静脉中精氨酸浓度的增加增强了PDV血流量，降低了PDV耗氧量，并有益地改变了猪的代谢特征，猪是一种用于研究人类营养和代谢的已建立的动物模型。
This study tested the hypothesis that an increase in arginine concentration in the portal vein may affect blood flow and oxygen consumption in the portal-drained viscera (PDV) of swine. Eight barrows (70 kg body weight) were surgically fitted with chronic catheters in the portal vein, ileal vein, and carotid artery. Thirteen days after the surgery, pigs that had been fasted for 12 h were randomly allocated to receive administration of either l-alanine (103 mg/kg body weight, isonitrogenous control) or l-arginine-HCl (61 mg/kg body weight) via the portal vein. Portal vein blood flow (PVBF) was measured with infusion of p-aminohippuric acid into the ileal vein, and blood samples were simultaneously obtained every 0.5 h for 4 h. Compared with the control, arginine infusion increased PVBF at 30-90 min after infusion but decreased PDV oxygen consumption at 60-150 min after infusion (P < 0.05). Plasma concentrations of glutamate at infusion times of 180-240 min and of arginine at infusion times of 60-240 min in arginine-infused pigs were higher than those for the control group (P < 0.05). Plasma concentrations of insulin and glucagon at the infusion times of 30-90 min were higher and of free fatty acids at the infusion times of 60-120 min were lower than those for the control pigs (P < 0.05). These results indicate that increasing arginine concentration in the portal vein enhances PDV blood flow, reduces PDV oxygen consumption, and beneficially alters the metabolic profile in swine, an established animal model for studying human nutrition and metabolism.