Isomer separation of polybrominated diphenyl ether metabolites using nanoESI-TIMS-MS
Isomer separation of polybrominated diphenyl ether metabolites using nanoESI-TIMS-MS
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DOI：
10.1007/s12127-016-0198-z
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发表时间：
2016-09-01
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通讯作者：
Fernandez-Lima, Francisco
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文献类型：
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作者：
Adams, Kendra J.;Montero, Dennise;Fernandez-Lima, Francisco
本文采用高分辨纳米电喷雾离子阱离子迁移谱-质谱联用技术（nESI-TIMS-MS）对羟基化多溴联苯醚（OH-PBDE）代谢产物进行了研究。特别是，实验离子-中性碰撞截面（CCS）的五个结构的OH-多溴二苯醚异构体使用TIMS-MS测量。候选结构提出了每个IMS带观察到的良好的一致性与实验CCS测量（5%的误差）。TIMS-MS对二元和三元混合物中OH-BDE结构异构体的基线和部分分离的分析能力显示为单电荷物质，R-IMS的迁移率分辨率类似于400。本研究为环境样品中低浓度OH-PBDE的分析提供了概念验证，该方法基于精确的碰撞截面和质量测量，无需衍生化和预分馏方案，从而显著降低了成本和分析时间。
In this paper, high-resolution nano-electrospray ionization-trapped ion mobility spectrometry coupled to mass spectrometry (nESI-TIMS-MS) is used for the study of hydroxylated polybrominated diphenyl ether (OH-PBDE) metabolites. In particular, experimental ion-neutral collision cross sections (CCS) were measured for five structural OH-PBDE isomers using TIMS-MS. Candidate structures were proposed for each IMS band observed in good agreement with the experimental CCS measurements (5 % error). The analytical power of TIMS-MS to baseline and partially separate structural isomers of OH-BDE in binary and ternary mixtures is shown for single charge species with a mobility resolving power of R-IMS similar to 400. This work provides the proof of concept for the analysis of low concentration OH-PBDE in environmental samples based on accurate collision cross section and mass measurements without the need for derivatization and pre-fractionation protocols, thus significantly reducing the cost and analysis time.