Sex-bias in utero alters ovarian reserve but not uterine capacity in female offspring
Sex-bias in utero alters ovarian reserve but not uterine capacity in female offspring
复制标题
子宫内的性别偏见会改变女性后代的卵巢储备功能，但不会改变子宫容量
DOI：
10.1101/2022.07.11.499550
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Geijer-Simpson A
中科院分区：
文献类型：
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作者：
Geijer-Simpson A
胎儿所暴露的环境压力会影响出生后后代的一系列生理功能。我们的目的是利用猪模型确定类固醇激素对雌性后代生殖潜力的子宫内影响。屠宰时收集母畜偏向（母畜占65%，n = 15）、非偏向（母畜占45% - 54.9%,n = 15）和公畜偏向（母畜占35%，n = 9）母猪的生殖道（95-115 kg）。对卵巢和子宫角进行H&E或免疫组织化学处理。使用Levene’s检验分析组内数据的变异性，同时使用r中的混合线性模型分析数据。在卵巢储备中，出生体重与性别比例的交互作用显著（P= 0.015），来自雄性偏倚窝的低出生体重猪的原始卵泡数量较高，而来自雌性偏倚窝的猪的趋势相反。性别偏见对子宫内膜腺的发育没有影响。较低的出生体重降低了子宫内膜腺体的比例（P= 0.045），并且在雄性偏向和雌性偏向的幼崽中变化更大（P= 0.026）。雄性偏倚窝的原始卵泡变异性大于非偏倚窝和雌性偏倚窝（P= 0.014）。同样，男性和女性偏倚的胎鼠子宫内膜间质核的范围比无偏倚的胎鼠大（P= 0.028）。一个重要的发现是，雄性偏向胎鼠的雌性卵巢的原始卵泡和雄性偏向胎鼠和雌性偏向胎鼠的子宫内膜基质细胞计数的差异更大。这可能会放大雄性偏向的幼崽中雌性繁殖成功率的可变性。
Environmental stressors to which a fetus is exposed affect a range of physiological functions in postnatal offspring. We aimed to determine the in utero effect of steroid hormones on the reproductive potential of female offspring using a porcine model. Reproductive tracts of pigs from female-biased (>65% female, n = 15), non-biased (45–54.9% female, n = 15), and male-biased litters (<35% females, n = 9) were collected at slaughter (95–115 kg). Ovaries and uterine horns were processed for H&E or immunohistochemistry. Variability of data within groups was analyzed with a Levene’s test, while data were analyzed using mixed linear models in R. In the ovarian reserve, there was a significant birth weight by sex ratio interaction (P= 0.015), with low birth weight pigs from male-biased litters having higher numbers of primordial follicles with opposite trends seen in pigs from female-biased litters. Sex bias held no effect on endometrial gland development. A lower birth weight decreased the proportion of glands found in the endometrium (P= 0.045) and was more variable in both male-biased and female-biased litters (P= 0.026). The variability of primordial follicles from male-biased litters was greater than non- and female-biased litters (P= 0.014). Similarly, endometrial stromal nuclei had a greater range in male- and female-biased litters than non-biased litters (P= 0.028). A crucial finding was the greater variability in primordial follicles in the ovaries from females derived from male-biased litters and stromal cell count in the endometrium of females from male- and female-biased litters. This could be inflating the variability of reproductive success seen in females from male-biased litters.