Ozone enhances the airway inflammation initiated by diesel exhaust
Ozone enhances the airway inflammation initiated by diesel exhaust
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DOI：
10.1016/j.rmed.2006.11.010
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发表时间：
2007-06-01
影响因子：
4.3
通讯作者：
Blomberg, Anders
中科院分区：
文献类型：
--
作者：
Bosson, Jenny;Pourazar, Jamshid;Blomberg, Anders
接触空气污染与不利的健康影响有关，颗粒物（PM）和臭氧（03）都显示出相当重要的影响。柴油发动机排气（DE）和03在气道中产生实质性的炎症效应。然而，尚未确定随后的03暴露是否会增加柴油诱导的气道炎症效应。健康受试者接受了两个单独的暴露系列：在pm浓度为300 μ g/m(3)的环境中暴露1小时，然后在5小时后分别暴露于过滤空气和0.2 ppm 03环境中2小时。第二次暴露后18 h采集诱导痰。DE+O-3处理后痰中性粒细胞（PMN）百分比和髓过氧化物酶（MPO）浓度显著高于DE+空气处理（p < 0.05和< 0.05）。MPO浓度与PMN细胞总数之间存在显著相关性（p = 0.001）， MPO与基质金属蛋白酶-9 （MMP-9）之间也存在显著相关性（p < 0.001）。与DE+空气相比，DE+O-3暴露后PMN和MPO显著增加，表明O-3诱导的DE诱导炎症放大。此外，MPO反应与pmn和MMP-9数量之间的相关性表明，pmn被激活，导致更强的炎症反应。目前的研究表明，O-3暴露显著增加了由柴油废气建立的炎症反应。在考虑空气污染对健康的影响时，应考虑到颗粒污染与O-3依次暴露之间的这种相互作用。(c) 2006 Elsevier Ltd.版权所有。
Exposure to air pollution is associated with adverse health effects, with particulate matter (PM) and ozone (03) both indicated to be of considerable importance. Diesel engine exhaust (DE) and 03 generate substantial inflammatory effects in the airways. However, as yet it has not been determined whether a subsequent 03 exposure would add to the diesel-induced airway inflammatory effects.Healthy subjects underwent two separate exposure series: A 1-h DE exposure at a PM-concentration of 300 mu g/m(3), followed after 5 h by a 2-h exposure to filtered air and 0.2 ppm 03, respectively. Induced sputum was collected 18 h after the second exposure.A significant increase in the percentage of neutrophils (PMN) and concentration of myeloperoxidase (MPO) was seen in sputum post DE+O-3 vs. DE+air (p < 0.05 and < 0.05, respectively). Significant associations were observed between the responses in MPO concentration and total PMN cells (p = 0.001), and also between MPO and matrix metalloproteinase-9 (MMP-9) (p < 0.001).The significant increase of PMN and MPO after the DE+O-3 exposures, compared to DE+air, denotes an O-3-induced magnification of the DE-induced inflammation. Furthermore, the correlation between responses in MPO and number of PMNs and MMP-9 illustrate that the PMNs are activated, resulting in a more potent inflammatory response.The present study indicates that O-3 exposure adds significantly to the inflammatory response that is established by diesel exhaust. This interaction between exposure to particulate pollution and O-3 in sequence should be taken into consideration when health effects of air pollution are considered. (c) 2006 Elsevier Ltd. All rights reserved.