Identification and characterization of 22 genes that affect the vulval cell lineages of the nematode Caenorhabditis elegans.
Identification and characterization of 22 genes that affect the vulval cell lineages of the nematode Caenorhabditis elegans.
复制标题
影响线虫外阴细胞谱系的 22 个基因的鉴定和表征。
DOI：
10.1093/genetics/110.1.17
复制
发表时间：
1985
期刊：
影响因子：
3.3
通讯作者：
Horvitz,HR
中科院分区：
文献类型：
--
作者：
Ferguson,EL;Horvitz,HR
在外阴细胞谱系中的线虫秀丽隐杆线虫的95个突变体已被分离，根据它们显示两种表型之一，外阴或Multivulva。在定义了12个基因的无外阴突变体中，不存在外阴。在定义了10个基因的多阴门突变体中，一个或多个额外的阴门样突起位于动物的腹侧沿着。一个单一的隐性突变是负责大多数，但不是全部，这些菌株的表型。这22个基因中有15个由多个等位基因代表。我们已经通过各种遗传标准表明，在22个基因中的6个中导致外阴无或多叶表型的突变最有可能消除基因功能。此外，其他七个基因中的Vulvaless或Multivava突变最有可能导致基因功能的部分降低;这些基因中任何一个的活性缺失都可能导致致死或不育。我们的研究结果表明，我们可能已经确定了这两类基因的大部分或全部。
Ninety-five mutants of the nematodeCaenorhabditis elegansaltered in the cell lineages of the vulva have been isolated on the basis of their displaying one of two phenotypes, Vulvaless or Multivulva. In Vulvaless mutants, which define 12 genes, no vulva is present. In Multivulva mutants, which define ten genes, one or more supernumerary vulva-like protrusions are located along the ventral side of the animal. A single recessive mutation is responsible for the phenotypes of most, but not all, of these strains. Fifteen of these 22 genes are represented by multiple alleles. We have shown by a variety of genetic criteria that mutations that result in a Vulvaless or Multivulva phenotype in six of the 22 genes most likely eliminate gene function. In addition, Vulvaless or Multivulva mutations in seven of the other genes most likely result in a partial reduction of gene function; the absence of the activity of any of these genes probably results in lethality or sterility. Our results suggest that we may have identified most, or all, genes of these two classes.