Human fatty acid synthesis is stimulated by a eucaloric low fat, high carbohydrate diet
Human fatty acid synthesis is stimulated by a eucaloric low fat, high carbohydrate diet
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DOI：
10.1172/jci118645
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发表时间：
1996-05-01
影响因子：
15.9
通讯作者：
Hirsch, J
中科院分区：
文献类型：
--
作者：
Hudgins, LC;Hellerstein, M;Hirsch, J
一种新的实验方法被用来确定无热量、低脂肪、高碳水化合物饮食是否会增加脂肪酸合成。正常志愿者连续 25 天食用低脂液体配方饮食（10% 卡路里为脂肪，75% 为葡萄糖聚合物，n = 7）或高脂肪饮食（40% 卡路里为脂肪，45% 为葡萄糖聚合物，n = 3）。每种饮食的脂肪酸组成与每个受试者的脂肪组织组成相匹配，并与极低密度脂蛋白甘油三酯的组成进行比较。到第 10 天，所有低脂饮食受试者的 VLDL 甘油三酯中棕榈酸酯显着增加，但亚油酸酯缺乏。新合成的脂肪酸占 VLDL 甘油三酯的 44+/-10%。对静脉注射 C-13-乙酸酯标记的棕榈酸酯的质量同位素分布分析证实，棕榈酸酯合成的增加可能是棕榈酸甘油三酯积累和亚油酸酯“稀释”的原因。相比之下，高脂肪饮食中的脂肪酸合成最少。因此，饮食中用碳水化合物替代脂肪会刺激脂肪酸合成以及富含棕榈酸酯、缺乏亚油酸酯的甘油三酯在血浆中的积累。这些变化可能会对心血管系统产生不利影响。
A new experimental approach was used to determine whether a eucaloric, low fat, high carbohydrate diet increases fatty acid synthesis. Normal volunteers consumed low fat liquid formula diets (10% of calories as fat and 75% as glucose polymers, n = 7) or high fat diets (40% of calories as fat and 45% as glucose polymers, n = 3) for 25 d. The fatty acid composition of each diet was matched to the composition of each subject's adipose tissue and compared with the composition of VLDL triglyceride. By day 10, VLDL triglyceride was markedly enriched in palmitate and deficient in linoleate in all subjects on the low fat diet. Newly synthesized fatty acids accounted for 44+/-10% of the VLDL triglyceride. Mass isotopomer distribution analysis of palmitate labeled with intravenously infused C-13-acetate confirmed that increased palmitate synthesis was the likely cause for the accumulation of triglyceride palmitate and ''dilution'' of linoleate. In contrast, there was minimal fatty acid synthesis on the high fat diet. Thus, the dietary substitution of carbohydrate for fat stimulated fatty acid synthesis and the plasma accumulation of palmitate-enriched, linoleate-deficient triglyceride. Such changes could have adverse effects on the cardiovascular system.