Small interfering RNA pathway modulates persistent infection of a plant virus in its insect vector.
Small interfering RNA pathway modulates persistent infection of a plant virus in its insect vector.
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小干扰RNA途径调节植物病毒在其昆虫载体中的持续感染
DOI：
10.1038/srep20699
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发表时间：
2016-02-11
影响因子：
4.6
通讯作者：
Wei T
中科院分区：
文献类型：
--
作者：
Lan H;Wang H;Chen Q;Chen H;Jia D;Mao Q;Wei T
Plant reoviruses, rhabdoviruses, tospoviruses, and tenuiviruses are transmitted by insect vectors in a persistent-propagative manner. How such persistent infection of plant viruses in insect vectors is established and maintained remains poorly understood. In this study, we used rice gall dwarf virus (RGDV), a plant reovirus, and its main vector leafhopperRecilia dorsalisas a virus–insect system to determine how the small interference (siRNA) pathway modulates persistent infection of a plant virus in its insect vector. We showed that a conserved siRNA antiviral response was triggered by the persistent replication of RGDV in cultured leafhopper cells and in intact insects, by appearance of virus-specific siRNAs, primarily 21-nt long, and the increased expression of siRNA pathway core components Dicer-2 and Argonaute-2. Silencing of Dicer-2 using RNA interference strongly suppressed production of virus-specific siRNAs, promoted viral accumulation, and caused cytopathological changesin vitroandin vivo. When the viral accumulation level rose above a certain threshold of viral genome copy (1.32 × 1014copies/μg insect RNA), the infection of the leafhopper by RGDV was lethal rather than persistent. Taken together, our results revealed a new finding that the siRNA pathway in insect vector can modulate persistent infection of plant viruses.
