G6PC3, ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers.
G6PC3, ALDOA and CS induction accompanies mir-122 down-regulation in the mechanical asphyxia and can serve as hypoxia biomarkers.
复制标题
G6PC3、ALDOA 和 CS 诱导伴随机械性窒息中 mir-122 的下调，可作为缺氧生物标志物。
DOI：
10.18632/oncotarget.12931
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发表时间：
2016-10-26
期刊：
影响因子：
--
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Zeng Y;Lv Y;Tao L;Ma J;Zhang H;Xu H;Xiao B;Shi Q;Ma K;Chen L
缺氧影响不同的细胞生物学过程。为了揭示缺氧对体内miRNA调控的动态影响，我们检测了机械性窒息病例与颅脑损伤和失血性休克病例的人脑和心脏标本中所有miRNA的表达水平。我们进一步验证了另外84例人类标本和大鼠模型中差异表达的miRNAs。我们发现，与颅脑损伤和失血性休克相比，在机械性窒息的情况下，mir-122在脑和心脏组织中显著下调，并且其假定的靶点G6 PC 3、ALDOA和CS增加。我们的数据表明，mir-122及其靶点G6 PC 3、ALDOA和CS在调节葡萄糖和能量代谢的缺氧反应中发挥作用，可以作为缺氧生物标志物。
Hypoxia influences different cellular biological processes. To reveal the dynamics of hypoxia's effects on miRNA regulation in vivo, we examined the expression levels of all miRNAs in human brain and heart specimens from cases of mechanical asphyxia compared with those from cases of craniocerebral injury and hemorrhagic shock. We further validated differently expressed miRNAs in another 84 human specimens and rat models. We found that mir-122 was significantly down-regulated and that its putative targets G6PC3, ALDOA and CS were increased in the brain and cardiac tissues in cases of mechanical asphyxia compared with craniocerebral injury and hemorrhagic shock. Our data indicate that mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers.