Pathogenesis investigation of miR-199-5p in oral submucous fibrosis based on bioinformatics analysis
Pathogenesis investigation of miR-199-5p in oral submucous fibrosis based on bioinformatics analysis
复制标题
基于生物信息学分析的miR-199-5p在口腔粘膜下纤维化中的发病机制研究
DOI：
10.1111/odi.13008
复制
发表时间：
2019
期刊：
影响因子：
3.8
通讯作者：
Jiang Canhua
中科院分区：
文献类型：
--
作者：
Yuan Yongxiang;Li Ning;Zeng Lijuan;Shen Zhengzhong;Jiang Canhua
目的纤维化疾病是导致患者痛苦和死亡的主要原因之一。然而，没有系统的调查已经进行了纤维化相关的genes.Materials和MethodsBy查询PubMed使用关键字“纤维化”和“基因”或“蛋白质”，我们确定了纤维化相关基因在过去的十年。采用MAS3.0软件进行生物信息学分析。结果共鉴定出1,310个与纤维化相关的基因，其中TGF-β1、CTGF、MMP 9、HSP 47和S1 P主要与纤维化器官相关。总共预测了244个细胞组分术语、595个分子功能术语、1，816个细胞组分术语和136个KEGG通路注释。选择miR-199 - 5 p作为关键miRNA，其在OSF中具有较高的水平。上调的miR-199 - 5 p与OSF持续时间和OSF组织学分级显著相关（分别为p= 0.028和0.012）。过表达的miR-199 - 5 p降低了颊部成纤维细胞的增殖并诱导了细胞凋亡。此外，颊黏膜成纤维细胞中miR-199 - 5 p的过表达促进了I型胶原（COL I）和III型胶原（COL III）的表达水平。结论这些结果表明纤维化相关基因与一系列复杂的机制有关。miR-199 - 5 p的特征可能为OSF的治疗策略提供重要线索。
ObjectivesFibrosis diseases are one of the leading causes of suffering and death. However, no systematic investigation has been carried out on fibrosis‐related genes.Materials and MethodsBy querying PubMed using keywords “fibrosis” and “gene” or “protein,” we identified fibrosis‐related genes in the last decade. Bioinformatics analysis was performed by MAS 3.0 software. Key miRNA was selected to assess its relationship with oral submucous fibrosis (OSF) and fibroblast functions.ResultsA total of 1,310 genes related to fibrosis were identified.TGF‐β1, CTGF, MMP9, HSP47, and S1Pwere found to be associated with mainly fibrotic organs. In total, 244 cellular components terms, 595 molecular function terms, 1,816 cellular component terms, and 136 KEGG pathway annotations were predicted. miR‐199‐5p was selected as the key miRNA, which has higher level in OSF. Upregulated miR‐199‐5p was significantly related to OSF duration and OSF histological grade (p= 0.028 and 0.012, respectively). Overexpressive miR‐199‐5p reduced proliferation and induced apoptosis in buccal fibroblasts. Additionally, expression levels of collagen I (COL I) and III (COL III) were promoted by overexpressive miR‐199‐5p in buccal fibroblasts.ConclusionThese results indicate that fibrosis‐related genes are related to a series of complex mechanisms. The characteristics of miR‐199‐5p may supply important clues for developing therapeutic strategy for OSF.