808 nm driven Nd3+-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging
808 nm driven Nd3+-sensitized upconversion nanostructures for photodynamic therapy and simultaneous fluorescence imaging
复制标题
用于光动力治疗和同时荧光成像的 808 nm 驱动 Nd3 敏化上转换纳米结构
DOI：
10.1039/c4nr04953e
复制
发表时间：
2015-01-01
期刊：
影响因子：
6.7
通讯作者：
Zhang, Hong
中科院分区：
文献类型：
--
作者：
Wang, Dan;Xue, Bin;Zhang, Hong
上转换纳米颗粒（UCNPs）的体内生物学应用优选在700-850 nm而不是980 nm处激发，这是由于水的吸收。最近的方法在构建强大的Nd 3+掺杂的UCNPs与808 nm的激发特性依赖于一个厚的Nd 3+敏化壳。然而，对于非常重要和流行的基于Frster共振能量转移（FRET）的应用，例如光动力疗法（PDT）或可切换生物传感器，这种类型的结构具有导致差的能量转移的限制。在本工作中，我们设计了一种NaYF 4：Yb/Ho@NaYF4：Nd@NaYF4核-壳-壳纳米结构。我们已经证明，这种最佳的结构平衡的鲁棒性的上转换发射和FRET效率为基础的FRET生物应用。该概念的证明被证明用于由808 nm光触发的HeLa细胞的光动力疗法和同时荧光成像，其中实现了低加热和高PDT功效。
The in vivo biological applications of upconversion nanoparticles (UCNPs) prefer excitation at 700-850 nm, instead of 980 nm, due to the absorption of water. Recent approaches in constructing robust Nd3+ doped UCNPs with 808 nm excitation properties rely on a thick Nd3+ sensitized shell. However, for the very important and popular Frster resonance energy transfer (FRET)-based applications, such as photodynamic therapy (PDT) or switchable biosensors, this type of structure has restrictions resulting in a poor energy transfer. In this work, we have designed a NaYF4:Yb/Ho@NaYF4:Nd@NaYF4 core-shell-shell nanostructure. We have proven that this optimal structure balances the robustness of the upconversion emission and the FRET efficiency for FRET-based bioapplications. A proof of the concept was demonstrated for photodynamic therapy and simultaneous fluorescence imaging of HeLa cells triggered by 808 nm light, where low heating and a high PDT efficacy were achieved.