Proteomic analysis of a matrix stone: a case report
Proteomic analysis of a matrix stone: a case report
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DOI：
10.1007/s00240-009-0213-5
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发表时间：
2009-12-01
影响因子：
--
通讯作者：
Monga, Manoj
中科院分区：
文献类型：
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作者：
Canales, Benjamin K.;Anderson, Lorraine;Monga, Manoj
基质结石是在肾集合系统内以软的粘蛋白质物质存在的放射性核素。广角X射线衍射（XRD）和扫描电子显微镜（SEM）后，我们均质化手术取出的基质石，提取和纯化蛋白质，并使用串联质谱分析样品的蛋白质组组成。使用ProteinPilot 2.0搜索所得光谱，并以>95%置信度报告鉴定的蛋白质。主要的XRD矿物分析是生物磷灰石，和SEM显示纤维状，网状层状含有细菌，细胞，和晶体材料。在鉴定的33种独特蛋白质中，90%以前在基质结石中没有报道，超过70%可能被认为是炎症性或防御性的。其他基质结石蛋白质组的表征，特别是来自非感染性人群的蛋白质组，可能会深入了解这种罕见结石的发病机制以及结晶结石内发生的矿物学过程。
Matrix stones are radiolucent bodies that present as soft muco-proteinaceous material within the renal collecting system. Following wide-angle X-ray diffraction (XRD) and scanning electron microscopy (SEM), we homogenized a surgically removed matrix stone, extracted and purified protein, and analyzed samples using tandem mass spectrometry for proteomic composition. Resulting spectra were searched using ProteinPilot 2.0, and identified proteins were reported with >95% confidence. Primary XRD mineral analysis was a biological apatite, and SEM revealed fibrous, net-like laminations containing bacterial, cellular, and crystalline material. Of the 33 unique proteins identified, 90% have not been previously reported within matrix stones and over 70% may be considered inflammatory or defensive in nature. Characterization of other matrix stone proteomes, in particular from non-infectious populations, may yield insights into the pathogenesis of this rare stone as well as the mineralogical process that occurs within crystalline calculi.