Mitochondria-mediated apoptosis in human lung cancer A549 cells by 4-methylsulfinyl-3-butenyl isothiocyanate from radish seeds.
Mitochondria-mediated apoptosis in human lung cancer A549 cells by 4-methylsulfinyl-3-butenyl isothiocyanate from radish seeds.
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萝卜种子中的 4-甲基亚磺酰基-3-丁烯基异硫氰酸酯介导人肺癌 A549 细胞线粒体凋亡。
DOI：
10.7314/apjcp.2014.15.5.2133
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Lian
中科院分区：
文献类型：
--
作者：
Nan Wang;Wei Wang;Po Huo;Caiqin Liu;Jianhcang Jin;Lian
萝卜（Raphanus sativus L.）中发现的4-甲基亚磺酰基-3-丁烯基异硫氰酸酯（MTBITC），是一种众所周知抗癌剂。本研究探讨MTBITC诱导人肺癌A549细胞凋亡的机制。我们的PI染色结果表明，MTBITC处理显着增加凋亡的亚G1分数以剂量依赖性的方式。通过检测线粒体膜电位（Δ Vm）的变化，RT-PCR检测凋亡相关基因mRNA的表达，caspase比色法检测caspase-3和caspase-9的活性，探讨MTBITC诱导细胞凋亡的机制。MTBITC处理通过下调Bcl-2/ Bax和Bcl-xL/Bax的比率以及caspase-3和-9的活化来降低线粒体膜电位。因此，线粒体途径和Bcl-2基因家族可能参与MTBITC诱导A549细胞凋亡的机制。
4-Methylsulfinyl-3-butenyl isothiocyanate (MTBITC) found in the radish (Raphanus sativus L.), is a well- known anticancer agent. In this study, the mechanisms of the MTBITC induction of cell apoptosis in human A549 lung cancer cells were investigated. Our PI staining results showed that MTBITC treatment significantly increased the apoptotic sub-G1 fraction in a dose-dependent manner. The mechanism of apoptosis induced by MTBITC was investigated by testing the change of mitochondrial membrane potential (Δψm), the expression of mRNAs of apoptosis-related genes by RT-PCR, and the activities of caspase-3 and -9 by caspase colorimetric assay. MTBITC treatment decreased mitochondrial membrane potential by down-regulating the rate of Bcl-2/ Bax and Bcl-xL/Bax, and activation of caspase-3 and -9. Therefore, mitochondrial pathway and Bcl-2 gene family could be involved in the mechanisms of A549 cell apoptosis induced by MTBITC.
影响因子：
5.2
作者：
J. Chiao;F. Chung;R. Kancherla;T. Ahmed;A. Mittelman;Conaway Cc
通讯作者：
J. Chiao;F. Chung;R. Kancherla;T. Ahmed;A. Mittelman;Conaway Cc
影响因子：
56.9
作者：
Kothakota, S;Azuma, T;Williams, LT
通讯作者：
Williams, LT