Independence of repressive histone marks and chromatin compaction during senescent heterochromatic layer formation.
Independence of repressive histone marks and chromatin compaction during senescent heterochromatic layer formation.
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DOI：
10.1016/j.molcel.2012.06.010
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发表时间：
2012-07-27
期刊：
影响因子：
16
通讯作者：
Narita, Masashi
中科院分区：
文献类型：
--
作者：
Chandra, Tamir;Kirschner, Kristina;Thuret, Jean-Yves;Pope, Benjamin D.;Ryba, Tyrone;Newman, Scott;Ahmed, Kashif;Samarajiwa, Shamith A.;Salama, Rafik;Carroll, Thomas;Stark, Rory;Janky, Rekin's;Narita, Masako;Xue, Lixiang;Chicas, Agustin;Nunez, Sabrina;Janknecht, Ralf;Hayashi-Takanaka, Yoko;Wilson, Michael D.;Marshall, Aileen;Odom, Duncan T.;Babu, M. Madan;Bazett-Jones, David P.;Tavare, Simon;Edwards, Paul A. W.;Lowe, Scott W.;Kimura, Hiroshi;Gilbert, David M.;Narita, Masashi
抑制性表观遗传标记的扩展与胚胎发育过程中的异染色质形成有关，但是该机制的一般适用性尚不清楚。在这里，我们表明，抑制性组蛋白的核重排将H3K9me3和H3K27me3标记为非重叠结构层中，表征了人类成纤维细胞中与衰老相关的杂色灶（SAHF）形成。但是，这些抑制性标记的全球景观在SAHF形成时保持不变，这表明在体细胞中，可以通过空间重新定位预先存在的抑制性组蛋白来形成异染色质。这种模型通过临时复制时机与随后的SAHF的分层结构和抑制性标记的全球景观的相关性加强，从而使我们能够整合显微镜和基因组信息。此外，SAHF结构的调节不会影响这些抑制性标记的占用率，反之亦然。这些实验表明，基因组的高阶异染色质形成和表观遗传重塑可能是离散的事件。
The expansion of repressive epigenetic marks has been implicated in heterochromatin formation during embryonic development, but the general applicability of this mechanism is unclear. Here we show that nuclear rearrangement of repressive histone marks H3K9me3 and H3K27me3 into nonoverlapping structural layers characterizes senescence-associated heterochromatic foci (SAHF) formation in human fibroblasts. However, the global landscape of these repressive marks remains unchanged upon SAHF formation, suggesting that in somatic cells, heterochromatin can be formed through the spatial repositioning of pre-existing repressively marked histones. This model is reinforced by the correlation of presenescent replication timing with both the subsequent layered structure of SAHFs and the global landscape of the repressive marks, allowing us to integrate microscopic and genomic information. Furthermore, modulation of SAHF structure does not affect the occupancy of these repressive marks, nor vice versa. These experiments reveal that high-order heterochromatin formation and epigenetic remodeling of the genome can be discrete events.
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影响因子：
10.5
作者：
Barradas, Marta;Anderton, Emma;Gil, Jesus
通讯作者：
Gil, Jesus
影响因子：
10.5
作者：
Agger, Karl;Cloos, Paul A. C.;Helin, Kristian
通讯作者：
Helin, Kristian
DOI：
10.1073/pnas.0408021101
发表时间：
2004-12-14
影响因子：
11.1
作者：
Chadwick, BP;Willard, HF
通讯作者：
Willard, HF
影响因子：
30.8
作者：
Kolasinska-Zwierz P;Down T;Latorre I;Liu T;Liu XS;Ahringer J
通讯作者：
Ahringer J
影响因子：
23.9
作者：
Cui K;Zang C;Roh TY;Schones DE;Childs RW;Peng W;Zhao K
通讯作者：
Zhao K