Sertoli Cells Modulate Testicular Vascular Network Development, Structure, and Function to Influence Circulating Testosterone Concentrations in Adult Male Mice.
Sertoli Cells Modulate Testicular Vascular Network Development, Structure, and Function to Influence Circulating Testosterone Concentrations in Adult Male Mice.
复制标题
DOI：
10.1210/en.2016-1156
复制
发表时间：
2016-06
期刊：
影响因子：
4.8
通讯作者：
Smith LB
中科院分区：
文献类型：
--
作者：
Rebourcet D;Wu J;Cruickshanks L;Smith SE;Milne L;Fernando A;Wallace RJ;Gray CD;Hadoke PW;Mitchell RT;O'Shaughnessy PJ;Smith LB
睾丸脉管系统形成复杂的网络，提供氧气、微量营养素和睾丸废物清除。脉管系统也有助于睾丸功能，因为它既是将促性腺激素递送到睾丸的途径，也是将T转运到靶器官的途径。支持细胞是否在生后生活中调节睾丸血管系统中发挥作用从未明确证明。在这项研究中，我们使用急性支持细胞消融和急性生殖细胞消融模型，以解决支持细胞是否积极影响成年睾丸血管结构和功能。我们的研究结果表明，支持细胞在支持睾丸血管结构中起着关键作用。支持细胞（和生殖细胞）或单独的生殖细胞导致睾丸大小类似的减少，但只有支持细胞的特定损失导致睾丸内总血管体积，血管分支数量和小微血管数量的减少;单独的生殖细胞损失对睾丸血管系统没有影响。睾丸脉管系统的这些扰动导致脉管系统和睾丸间质组织之间的液体交换减少，这降低了促性腺激素刺激的循环T浓度，表明睾丸间质细胞刺激减少和/或T向脉管系统中的分泌减少。这些发现描述了一种新的范式，通过这种范式，激素和其他因素进出睾丸的运输可能受到支持细胞的影响，并强调这些细胞是增强这种内分泌关系的潜在靶点。
The testicular vasculature forms a complex network, providing oxygenation, micronutrients, and waste clearance from the testis. The vasculature is also instrumental to testis function because it is both the route by which gonadotropins are delivered to the testis and by which T is transported away to target organs. Whether Sertoli cells play a role in regulating the testicular vasculature in postnatal life has never been unequivocally demonstrated. In this study we used models of acute Sertoli cell ablation and acute germ cell ablation to address whether Sertoli cells actively influence vascular structure and function in the adult testis. Our findings suggest that Sertoli cells play a key role in supporting the structure of the testicular vasculature. Ablating Sertoli cells (and germ cells) or germ cells alone results in a similar reduction in testis size, yet only the specific loss of Sertoli cells leads to a reduction in total intratesticular vascular volume, the number of vascular branches, and the numbers of small microvessels; loss of germ cells alone has no effect on the testicular vasculature. These perturbations to the testicular vasculature leads to a reduction in fluid exchange between the vasculature and testicular interstitium, which reduces gonadotropin-stimulated circulating T concentrations, indicative of reduced Leydig cell stimulation and/or reduced secretion of T into the vasculature. These findings describe a new paradigm by which the transport of hormones and other factors into and out of the testis may be influenced by Sertoli cells and highlights these cells as potential targets for enhancing this endocrine relationship.