Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea
Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea
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DOI：
10.1099/ijsem.0.005004
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发表时间：
2021-01-01
影响因子：
2.8
通讯作者：
Wang, Xiangjing
中科院分区：
文献类型：
--
作者：
Jiang, Mengqi;Xu, Xi;Wang, Xiangjing
真菌病原体灰霉菌是许多经济上重要的水果、蔬菜和花卉中破坏性灰霉菌病的病原体，在世界范围内造成严重的经济损失。本研究分离到一株具有抑菌活性的放线菌NEAU- LD23(T)，并利用多相法对其进行了分类鉴定。根据基因型、表型和化学分类资料，该菌株是链霉菌属的一个新种，并建议将其命名为Streptomyces botrytidirefrens sp. 11。型应变为NEAU-LD23(T) (=CCTCC AA 2019029（T)=DSM 109824(T)）。此外，菌株NEAU- LD23(T)对葡萄球菌（B. cinerea）有较强的拮抗作用（82.6 +/- 2.5%），对其他9种植物病原真菌均有不同程度的抑制作用。菌株NEAU- LD23(T)菌丝的无细胞滤液和甲醇提取物均能显著抑制灰葡萄球菌菌丝的生长。为初步探讨其抗真菌作用机制，对菌株NEAU- LD23(T)进行了基因组测序和分析。AntiSMASH分析鉴定出了几个负责生物合成具有抗真菌活性的生物活性次级代谢物的基因簇，包括9-甲基streptimidone， echosides, anisomycin， coelichelin和去铁胺B.总之，本研究为我们提供了一个具有相当潜力用于番茄灰霉病生物防治的优秀菌株。
The fungal pathogen Botrytis cinerea is the causal agent of devastating gray mold diseases in many economically important fruits, vegetables, and flowers, leading to serious economic losses worldwide. In this study, a novel actinomycete NEAU- LD23(T) exhibiting antifungal activity against B. cinerea was isolated, and its taxonomic position was evaluated using a polyphasic approach. Based on the genotypic, phenotypic and chemotaxonomic data, it is concluded that the strain represents a novel species within the genus Streptomyces, for which the name Streptomyces botrytidirepellens sp. nov. is proposed. The type strain is NEAU-LD23(T) (=CCTCC AA 2019029(T)=DSM 109824(T)). In addition, strain NEAU- LD23(T) showed a strong antagonistic effect against B. cinerea (82.6 +/- 2.5%) and varying degrees of inhibition on nine other phytopathogenic fungi. Both cell-free filtrate and methanol extract of mycelia of strain NEAU- LD23(T) significantly inhibited mycelial growth of B. cinerea. To preliminarily explore the antifungal mechanisms, the genome of strain NEAU- LD23(T) was sequenced and analyzed. AntiSMASH analysis led to the identification of several gene clusters responsible for the biosynthesis of bioactive secondary metabolites with antifungal activity, including 9-methylstreptimidone, echosides, anisomycin, coelichelin and desferrioxamine B. Overall, this research provided us an excellent strain with considerable potential to use for biological control of tomato gray mold.