Identification and localization of insulin-like growth factor-binding protein (IGFBP) messenger RNAs in human hair follicle dermal papilla
Identification and localization of insulin-like growth factor-binding protein (IGFBP) messenger RNAs in human hair follicle dermal papilla
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DOI：
10.1111/1523-1747.ep12343649
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发表时间：
1996-03-01
影响因子：
6.5
通讯作者：
Werther, GA
中科院分区：
文献类型：
--
作者：
Batch, JA;Mercuri, FA;Werther, GA
胰岛素样生长因子 (IGF) 在毛囊生物学中的作用最近已得到认识，尽管迄今为止尚未确定其作用、产生位点以及胰岛素样生长因子结合蛋白 (IGFBP) 的调节。 IGF-I 对于正常毛发生长和发育至关重要，并且可能对毛发生长周期的调节很重要。在许多培养系统中，IGF-I 的作用受到 IGFBP 的调节。因此，如果 IGFBP 在人类毛囊中产生，它们可能在将 IGF-I 靶向其受体方面发挥作用，或者可能通过与基质蛋白相互作用来调节 IGF-I 的作用。我们使用原位杂交来定位生长期毛囊中 6 个 IGFBP 的信使 RNA，制备了 IGFBP（IGFBP-1 至 -6）的反义和正义 RNA 探针，并对成人面部皮肤的 5 μm 切片进行了探测。鉴定了 IGFBP-3、-4 和 -5 的信使 RNA，其中主要在真皮乳头中发现了 IGFBP-3 和 -5 mRNA，并在较小程度上发现了 IGFBP-4 mRNA。 IGFBP-C mRNA 也在真皮乳头/上皮基质边界被发现，IGFBP-4 和-5 的信使 RNA 也在毛囊周围的真皮鞘中被发现。 IGFBP-1、-2 和 -6 的信使 RNA 尚未鉴定。这些研究证明了 IGFBP mRNA 在毛囊中的特定定位，表明它们各自在毛发生长周期期间 IGF 作用的局部调节中发挥特定作用。
The role of the insulin-like growth factors (IGFs) in hair follicle biology has recently been recognized, although their actions, sites of production, and modulation by the insulin-like growth factor-binding proteins (IGFBPs) have not to date been defined. IGF-I is essential for normal hair growth and development, and may be important in regulation of the hair growth cycle, In many culture systems, IGF-I actions are modulated by the IGFBPs. Thus, if IGFBPs are produced in the human hair follicle, they may play a role in targeting IGF-I to its receptor or may modulate IGF-I action by interaction with matrix proteins. We have used in situ hybridization to localize messenger RNA for the six IGFBPs in anagen hair follicles, Anti-sense and sense RNA probes for the IGFBPs (IGFBP-1 to -6) were produced, and 5-mu m sections of adult facial skin were probed. Messenger RNA for IGFBP-3, -4, and -5 were identified, with predominantly IGFBP-3 and -5 mRNA found in the dermal papilla, and to a lesser extent IGFBP-4 mRNA. IGFBP-C mRNA was also found at the dermal papilla/epithelial matrix border, Messenger RNAs for both IGFBP-4 and -5 were also demonstrated in the dermal sheath surrounding the hair follicle. Messenger RNAs for IGFBP-1, -2, and -6 were not identified, These studies demonstrate specific localization of IGFBP mRNAs in hair follicles, suggesting that they each play specific roles in the local modulation of IGF action during the hair growth cycle.