A comprehensive evaluation of cattle introgression into US federal bison herds
A comprehensive evaluation of cattle introgression into US federal bison herds
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DOI：
10.1093/jhered/esl051
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发表时间：
2007-01-01
影响因子：
3.1
通讯作者：
Derr, James N.
中科院分区：
文献类型：
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作者：
Halbert, Natalie D.;Derr, James N.
遗传渐渗，特别是种间杂交，是世界范围内物种保护的一个重大威胁。在这项研究中，11个美国联邦野牛人口进行了全面检查的证据，线粒体和核家牛（牛）渐渗。线粒体基因渗入使用已建立的聚合酶链反应方法进行检查，并通过D-环序列分析确认。通过对野牛和家牛的微卫星电型和测序差异的检查，在14个染色体区域中评估了核渗入。只有一个群体被鉴定为家牛线粒体DNA渐渗。相反，在7个（63.6%）被检查的群体中发现了核渗入的证据。野牛在种群间转移的历史记载得到了DNA渗入传播证据的证实。虽然在大提顿国家公园、萨利山国家禁猎区、风洞国家公园或黄石国家公园的野牛中均未检测到细胞核或线粒体家养牛基因渗入，但仅从最后2个种群中获得了足够的样本量，以允许细胞核基因渗入检测限的统计学置信度（> 90%）。遗传独特的和不受干扰的人口的识别是至关重要的物种保护工作，这项研究作为一个模型的种间渗入的通用评估。
Genetic introgression, especially from interspecies hybridization, is a significant threat to species conservation worldwide. In this study, 11 US federal bison populations were comprehensively examined for evidence of both mitochondrial and nuclear domestic cattle (Bos taurus) introgression. Mitochondrial introgression was examined using established polymerase chain reaction methods and confirmed through analysis of D-loop sequences. Nuclear introgression was assessed in 14 chromosomal regions through examination of microsatellite electromorph and sequenced differences between bison and domestic cattle. Only one population was identified with domestic cattle mitochondrial DNA introgression. In contrast, evidence of nuclear introgression was found in 7 (63.6%) of the examined populations. Historic accounts of bison transfers among populations were corroborated with evidence of introgressed DNA transmission. While neither nuclear nor mitochondrial domestic cattle introgression was detected in bison from Grand Teton National Park, Sully's Hill National Game Preserve, Wind Cave National Park, or Yellowstone National Park, adequate sample sizes were available only from the last 2 populations to allow for statistical confidence (> 90%) in nuclear introgression detection limits. The identification of genetically unique and undisturbed populations is critical to species conservation efforts, and this study serves as a model for the generic evaluation of interspecies introgression.