Correlative transmission electron microscopy examination of nondemineralized and demineralized resin-dentin interfaces formed by two dentin adhesive systems.
Correlative transmission electron microscopy examination of nondemineralized and demineralized resin-dentin interfaces formed by two dentin adhesive systems.
复制标题
由两种牙本质粘合剂系统形成的非脱矿和脱矿树脂-牙本质界面的相关透射电子显微镜检查。
DOI：
10.1177/00220345960750030401
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发表时间：
1996
影响因子：
7.6
通讯作者：
Guerrero,D
中科院分区：
文献类型：
--
作者：
VanMeerbeek,B;ConnJr,LJ;Duke,ES;Eick,JD;Robinson,SJ;Guerrero,D
通过透射电子显微镜（TEM）对两种牙本质粘接剂Optibond （OPTI, Kerr）和Scotchbond MultiPurpose （SBMP, 3M）形成的树脂-牙本质界面进行超微形貌观察。非脱矿和脱矿切片的超微结构信息相互关联。假设两种粘合剂的不同化学配方会导致杂化层的不同形态外观。超微结构TEM检测证明，两种牙本质粘接剂体系均能在牙本质与树脂之间建立微力学结合，形成杂化层。然而，产生这种树脂-牙本质键的界面杂交过程似乎与两种体系的化学组成和应用方式有关。OPTI始终呈现出具有相对均匀的超微结构、电子密度和耐酸性能的杂化层。这三个参数对SBMP形成的杂化层变化较大。SBMP的特点是在杂化层的外表面发现了非晶相。尽管所研究的两种粘合剂系统都遵循全蚀刻概念，但它们特定的化学配方导致不同的界面超微结构，这可能与不同的潜在键合机制有关。然而，这些形态学发现的临床意义尚不清楚。
The resin-dentin interface formed by two dentin adhesives, Optibond (OPTI, Kerr) and Scotchbond MultiPurpose (SBMP, 3M), was ultramorphologically examined by transmission electron microscopy (TEM). Ultrastructural information from nondemineralized and demineralized sections was correlated. It was hypothesized that the different chemical formulations of the two adhesives would result in a different morphological appearance of the hybrid layer. Ultrastructural TEM examination proved that each of the two dentin adhesive systems was able to establish a micromechanical bond between dentin and resin with the formation of a hybrid layer. However, the interfacial hybridization process that took place to produce this resin-dentin bond appeared to be specifically related to the chemical composition and application modes of both systems. OPTI consistently presented with a hybrid layer with a relatively uniform ultrastructure, electron density, and acid resistance. These three parameters were found to be more variable for the hybrid layer formed by SBMP. Characteristic of SBMP was the identification of an amorphous phase deposited at the outer surface of the hybrid layer. Although both adhesive systems investigated follow a total-etch concept, their specific chemical formulations result in different interfacial ultrastructures that are probably related to different underlying bonding mechanisms. The clinical significance of these morphological findings, however, is still unknown.