Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization
Structural characterization of oligosaccharides from free radical depolymerized fucosylated glycosaminoglycan and suggested mechanism of depolymerization
复制标题
自由基解聚岩藻糖胺聚糖寡糖的结构表征和解聚机制
DOI：
10.1016/j.carbpol.2021.118368
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发表时间：
2021-07-20
影响因子：
11.2
通讯作者：
Zhao, Jinhua
中科院分区：
文献类型：
--
作者：
Chen, Ru;Yin, Ronghua;Zhao, Jinhua
自由基解聚是多糖结构分析中常用的方法，其主要挑战是裂解位点的分析和新形成末端的表征。在这里，来自H.用H2 O2对褐斑藻（HfFG）进行解聚，得到一系列寡糖，并对其结构进行了鉴定。对于三聚体的非还原末端是完整的GalNAc（4S 6S），切割位点应主要是FG骨架中GlcA和GalNAc之间的β（1，3）键。同时，二糖和三糖的还原端几乎是GlcA的二羧酸衍生物，可能是由于还原端GlcA的C-C键被氧化断裂而产生的。此外，位于还原端的D-GalNAc-beta（1，4）-D-GlcA和L-FucS-alpha（1，3）-D-GlcA中的糖苷键可以被切割，并且释放的GalNAc（4S 6S）被氧化为N-乙酰半乳糖胺酸。
Free radical depolymerization is a common method in structural analysis of polysaccharides, the major challenge is the analysis of the cleavage site and characterization of newly formed ends in this reaction. Here, a fucosylated glycosaminoglycan from H. fuscopunctata (HfFG) was depolymerized by H2O2 and a series of oligosaccharides were purified and their structures were elucidated. For non-reducing ends of the trisaccharides were intact GalNAc(4S6S), the cleavage site should mainly be the beta(1,3) linkages between GlcA and GalNAc in the backbone of FG. Meanwhile, the reducing ends of the disaccharides and trisaccharides were almost dicarboxylic acid derivatives of GlcA, possibly arising from oxidative breaking of the C-C bond of GlcA at the reducing ends. In addition, glycosidic linkages in D-GalNAc-beta(1,4)-D-GlcA and L-FucS-alpha(1,3)-D-GlcA located at the reducing end could be cleaved, and the released GalNAc(4S6S) were oxidized to N-acetylgalactosaminic acid.