Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population
Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population
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DOI：
10.1046/j.1365-313x.1998.00115.x
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发表时间：
1998-04-01
期刊：
影响因子：
7.2
通讯作者：
Kuiper, MTR
中科院分区：
文献类型：
--
作者：
Alonso-Blanco, C;Peeters, AJM;Kuiper, MTR
已为新的兰茨贝格直立/佛得角群岛 (Ler/Cvi) 重组自交系 (RIL) 群体生成了基于扩增片段多态性 (AFLP) 的连锁图谱。总共 321 个基于分子 PCR 的标记和直立突变被绘制。还对兰茨伯格直立/哥伦比亚 (Ler/Col) RIL 群体中的 AFLP 标记进行了分析（Lister 和 Dean，1993），并且 395 个 AFLP 标记已整合到先前的 122 个 RFLP、CAPS 和 SSLP 的拟南芥分子图谱中。这使得能够评估 AFLP 技术在拟南芥连锁分析中的效率和稳健性。在整个连锁图中发现了 AFLP 标记。两个 RIL 图谱可以通过 49 个共同标记进行整合，这些标记都映射在相似的位置。比较这两个图谱得出的结论是，来自共同亲本的分离带可以在不同群体之间进行比较，并且分子大小相似的 AFLP 带在两个不同生态型中使用相同的引物组合扩增，很可能对应于相同的基因座。发现 AFLP 聚集在着丝粒区域周围，作者通过使用三体植物对 AFLP 带进行定量分析，确定了 3 号染色体着丝粒的图谱位置。 AFLP标记也用于估计三个生态型之间的多态性率。与 Ler 和 Col 相比，Ler 和 Cvi 之间发现的多态性率更大，这意味着新的 RIL 群体将为绘制 DNA 多态性和数量性状位点图谱提供有用的材料。
An amplified fragment polymorphism (AFLP) based linkage map has been generated for a new Landsberg erecta/Cape Verde Islands (Ler/Cvi) recombinant inbred line (RIL) population. A total of 321 molecular PCR based markers and the erects mutation were mapped. AFLP markers were also analysed in the Landsberg erecta/Columbia (Ler/Col) RIL population (Lister and Dean, 1993) and 395 AFLP markers have been integrated into the previous Arabidopsis molecular map of 122 RFLPs, CAPSs and SSLPs. This enabled the evaluation of the efficiency and robustness of AFLP technology for linkage analyses in Arabidopsis. AFLP markers were found throughout the linkage map. The two RIL maps could be integrated through 49 common markers which all mapped at similar positions. Comparison of both maps led to the conclusion that segregating bands from a common parent can be compared between different populations, and that AFLP bands of similar molecular size, amplified with the same primer combination in two different ecotypes, are likely to correspond to the same locus. AFLPs were found clustering around the centromeric regions, and the authors have established the map position of the centromere of chromosome 3 by a quantitative analysis of AFLP bands using trisomic plants. AFLP markers were also used to estimate the polymorphism rate among the three ecotypes. The larger polymorphism rate found between Ler and Cvi compared to Ler and Col will mean that the new RIL population will provide a useful material to map DNA polymorphisms and quantitative trait loci.