A System for Monocytic Differentiation of Leukemic Cells HL 60 by a Short Exposure to 1,25-Dihydroxycholecalciferol
A System for Monocytic Differentiation of Leukemic Cells HL 60 by a Short Exposure to 1,25-Dihydroxycholecalciferol
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通过短时间接触 1,25-二羟基胆钙化醇使白血病细胞 HL 60 单核细胞分化的系统
DOI：
10.3181/00379727-179-42098
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发表时间：
1985
影响因子：
--
通讯作者：
Z. Brelvi
中科院分区：
文献类型：
--
作者：
G. P. Studzinski;A. Bhandal;Z. Brelvi
摘要人早幼粒细胞系HL 60可被多种诱导剂诱导向更成熟的髓系或单核系分化。该过程被认为需要暴露于诱导剂数天，因此难以鉴定对分化程序至关重要的早期细胞变化，并且难以将诱导与细胞周期的阶段相关联。为了研究白血病细胞分化的动力学，我们开发了一种诱导HL 60细胞亚群中单核细胞快速成熟的系统。将细胞在无血清培养基中暴露于10-7 M 1，25-二羟基胆钙化醇4小时。随后在完全培养基中孵育，导致从暴露于诱导剂后10小时开始，通过几个标准（吞噬作用、非特异性酯酶反应、粘附基质、细胞形态）可识别细胞分化。大约20小时后，培养物中30-40%的细胞显示分化的表型，并且能够吞噬。此后，培养物中分化细胞的比例降低。该系统已被用于研究c-myc癌基因表达与成熟动力学的关系，发现该基因表达的抑制先于表型分化开始约8小时，是短暂的，并伴随着细胞增殖的短暂阻滞，其在暴露于诱导剂的24小时内恢复正常速率。
Abstract The human promyelocytic cell line HL 60 can be induced to differentiate toward more mature myeloid or monocytic forms by a variety of agents. This process is thought to require several days of exposure to the inducer, thus making it difficult to identify the early cellular changes which are fundamental to the differentiation program, and to relate the induction to phases of the cell cycle. In order to study the kinetics of leukemic cell differentiation we have developed a system for the induction of rapid monocytic maturation in a subpopulation of HL 60 cells. The cells are exposed to 10-7 M 1,25-dihydroxycholecalciferol for 4 hr in serum-free medium. Subsequent incubation in a complete medium results in cellular differentiation recognizable by several criteria (phagocytosis, nonspecific esterase reaction, adherence to substratum, cell morphology) beginning at 10 hr from the exposure to the inducer. Approximately 20 hr later 30-40% of the cells in culture show the differentiated phenotype and are capable of phagocytosis. The proportion of differentiated cells in culture decreases thereafter. This system has been utilized to study the expression of c-myc oncogene in relation to the kinetics of maturation, and it was found that the inhibition of the expression of this gene precedes the onset of phenotypic differentiation by approximately 8 hr, is transient, and is accompanied by a brief retardation of cell proliferation, which resumes the normal rate within 24 hr of the exposure to the inducer.
疱疹病毒的胸苷激酶作为分离和表征未知哺乳动物启动子和增强子的载体。
DOI：
--
发表时间：
1984
期刊：
Journal of molecular and applied genetics
影响因子：
--
作者：
Pater,MM;Pater,A
通讯作者：
Pater,A
细胞周期事件与 6-硫鸟嘌呤对 HL-60 细胞的细胞毒性和分化诱导作用终止增殖相关。
影响因子：
11.2
作者：
Schwartz,EL;Blair,OC;Sartorelli,AC
通讯作者：
Sartorelli,AC