Interaction between submicron COD crystals and renal epithelial cells.
Interaction between submicron COD crystals and renal epithelial cells.
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DOI：
10.2147/ijn.s33848
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发表时间：
2012
影响因子：
8
通讯作者：
Yang RE
中科院分区：
文献类型：
--
作者：
Peng H;Ouyang JM;Yao XQ;Yang RE
本研究旨在观察大小为150 ± 50 nm的亚微米二水草酸钙（COD）与非洲绿色猴肾上皮细胞（Vero细胞）损伤前后的粘附特性，探讨肾结石形成的机制。用过氧化氢氧化损伤Vero细胞，建立细胞损伤模型。用扫描电镜观察Vero-COD粘附情况。采用电感耦合等离子体发射光谱法定量测定了COD微晶的附着量。采用纳米粒度分析仪和激光扫描共聚焦显微镜分别检测Vero细胞表面zeta电位的变化和粘附过程中骨桥蛋白表达的变化。通过测量粘附过程中丙二醛含量和细胞活力的变化来评估细胞损伤水平。损伤组Vero细胞对COD微晶的粘附能力明显强于对照组。在粘附到COD后，细胞活力下降，丙二醛含量和细胞表面zeta电位增加，并且骨桥蛋白的荧光强度降低，因为骨桥蛋白分子被COD成功地覆盖。亚微米COD在粘附过程中进一步破坏细胞，特别是对照组中的Vero细胞，导致附着的微晶量升高。亚微米COD可在粘附过程中进一步损伤受损的Vero细胞。附着的微晶的数量与细胞损伤的程度成正比。粘附在受损上皮细胞上的微晶数量增加在早期肾结石的形成中起重要作用。
This study aims to investigate the adhesion characteristics between submicron calcium oxalate dihydrate (COD) with a size of 150 ± 50 nm and African green monkey kidney epithelial cells (Vero cells) before and after damage, and to discuss the mechanism of kidney stone formation. Vero cells were oxidatively injured by hydrogen peroxide to establish a model of injured cells. Scanning electron microscopy was used to observe Vero–COD adhesion. Inductively coupled plasma emission spectrometry was used to quantitatively measure the amount of adhered COD microcrystals. Nanoparticle size analyzer and laser scanning confocal microscopy were performed to measure the change in the zeta potential on the Vero cell surface and the change in osteopontin expression during the adhesion process, respectively. The level of cell injury was evaluated by measuring the changes in malonaldehyde content, and cell viability during the adhesion process. The adhesion capacity of Vero cells in the injury group to COD microcrystals was obviously stronger than that of Vero cells in the control group. After adhesion to COD, cell viability dropped, both malonaldehyde content and cell surface zeta potential increased, and the fluorescence intensity of osteopontin decreased because the osteopontin molecules were successfully covered by COD. Submicron COD further damaged the cells during the adhesion process, especially for Vero cells in the control group, leading to an elevated amount of attached microcrystals. Submicron COD can further damage injured Vero cells during the adhesion process. The amount of attached microcrystals is proportional to the degree of cell damage. The increased amount of microcrystals that adhered to the injured epithelial cells plays an important role in the formation of early-stage kidney stones.