MicroRNAs from the same precursor have different targeting properties.
MicroRNAs from the same precursor have different targeting properties.
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DOI：
10.1186/1758-907x-3-8
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发表时间：
2012-09-27
期刊：
影响因子：
--
通讯作者：
Griffiths-Jones S
中科院分区：
文献类型：
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作者：
Marco A;Macpherson JI;Ronshaugen M;Griffiths-Jones S
MicroRNA的加工产生了来自前体发夹的两个臂(5‘和3’)的两个潜在成熟产物的中间双链。通常认为，一个序列被降解，另一个序列被掺入到RNA诱导的沉默复合体中。然而，这两个前体臂都可能产生成熟microRNA的功能水平，并且主要产物可能会因物种的不同、组织的不同或发育阶段的不同而变化。因此，前体的两个臂都有可能产生功能成熟的microRNAs。我们研究了来自相同前microRNAs的5‘和3’臂的成熟序列预测的mRNA靶标之间的关系。使用六种最先进的靶预测算法，我们发现5‘/3’microRNA对针对mRNAs的3‘非翻译区中的不同位点。我们还发现，这些对通常不针对重叠的基因集，或功能相关的基因。我们表明，由相同前体microRNAs产生的替代成熟产物具有不同的靶向特性，因此具有不同的生物学功能。这些数据有力地表明，手臂选择的发育或进化变化将产生重大的功能后果。
The processing of a microRNA results in an intermediate duplex of two potential mature products that derive from the two arms (5′ and 3′) of the precursor hairpin. It is often suggested that one of the sequences is degraded and the other is incorporated into the RNA-induced silencing complex. However, both precursor arms may give rise to functional levels of mature microRNA and the dominant product may change from species to species, from tissue to tissue, or between developmental stages. Therefore, both arms of the precursor have the potential to produce functional mature microRNAs. We have investigated the relationship between predicted mRNA targets of mature sequences derived from the 5′ and 3′ arms of the same pre-microRNAs. Using six state-of-the-art target prediction algorithms, we find that 5′/3′ microRNA pairs target different sites in 3′ untranslated regions of mRNAs. We also find that these pairs do not generally target overlapping sets of genes, or functionally related genes. We show that alternative mature products produced from the same precursor microRNAs have different targeting properties and therefore different biological functions. These data strongly suggest that developmental or evolutionary changes in arm choice will have significant functional consequences.