PSV-B-17 Effect of Dietary Supplementation with Vitamins/Minerals and/or Energy on Fetoplacental Vascularity in Crossbred Angus Heifers
PSV-B-17 Effect of Dietary Supplementation with Vitamins/Minerals and/or Energy on Fetoplacental Vascularity in Crossbred Angus Heifers
复制标题
PSV-B-17 膳食补充剂维生素/矿物质和/或能量对杂交安格斯小母牛胎儿胎盘血管的影响
DOI：
10.1093/jas/skac247.634
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发表时间：
2022
影响因子：
3.3
通讯作者：
L. Reynolds
中科院分区：
文献类型：
--
作者：
Bethania J Davila Ruiz;C. Dahlen;Jennifer L Hurlbert;F. Baumgaertner;K. Bochantin;A. C. B. Menezes;W. Diniz;J. Kirsch;P. Borowicz;S. Cánovas;L. Reynolds
这些研究的目的是评价妊娠期间补充维生素和矿物质（VTM）和/或能量（NRG）是否会影响杂交安格斯小母牛胎盘胎儿部分（称为子叶（COT））的血管分布。我们假设饮食中补充VTM和/或NRG会改善胎儿胎盘血管发育，这是胎盘功能的重要指标。进行了两个实验：在Exp. 1、34头小母牛在人工授精（AI）前71天被随机分配到VTM/NoVTM补充组。在育种时，维持饮食，并将小母牛随机分配接受NRG，产生以下处理组合：NoVTM-NoNRG（n=8）、NoVTM-NRG（n=8）、VTM-NoNRG（n=9）和VTM-NRG（n=9）。维持饮食补充直到第83天，此时小母牛被卵巢切除并收集COT。在Exp. 2.在育种时，将72头小母牛随机分配至VTM补充（n=36）或对照（CON）（n=36）治疗组，并持续至分娩，此时收集来自28头小母牛的亚组的COT。使用兔抗-CD 34和抗-CD 31抗体作为血管分布的标志物，通过免疫组织化学评价来自两个实验的COT，沿着使用DAPI进行背景核染色。在d 83组中，BS 1凝集素被用作胎儿胎盘组织的标记物。采集每只动物的三张图像，并分析组织区域内血管的百分比/数量。采用SAS的GLM程序对数据进行分析。结果表明，在第83天，COT血管分布不受VTM（P值= 0.5030）、NRG（P值= 0.5450）或其相互作用（P值= 0.6664）的影响，而在分娩时，VTM中COT血管分布有大于CON的趋势（P值=0.0723）。这些结果表明，从育种到分娩的饮食VTM补充可能对杂交安格斯小母牛的胎盘血管发育有有益的影响。
The objective of these studies was to evaluate if vascularity of the fetal part of the placenta, termed cotyledon (COT), of crossbreed Angus heifers is affected by vitamin and mineral (VTM) and/or energy (NRG) supplementation during pregnancy. We hypothesized that dietary supplementation with VTM and/or NRG would improve fetoplacental vascular development, which is an important index of placental function. Two experiments were conducted: In Exp. 1, 34 heifers were randomly assigned to VTM/NoVTM supplementation 71 days before artificial insemination (AI). At breeding, the diet was maintained and heifers were randomly assigned to receive NRG, resulting in the following treatments combinations: NoVTM-NoNRG (n=8), NoVTM-NRG (n=8), VTM-NoNRG (n=9), and VTM-NRG (n=9). Dietary supplementation was maintained until d 83 when heifers were ovariohysterectomized and COT was collected. On Exp. 2, 72 heifers were randomly assigned to VTM supplementation (n=36) or control (CON) (n=36) treatments at breeding and continuing until parturition when COT from a subset of 28 heifers was collected. The COT from both experiments was evaluated by immunohistochemistry using rabbit anti-CD34 and anti-CD31 antibodies as markers for vascularity along with DAPI for background nuclear staining. In the d 83 group, BS1 lectin was used as a marker of fetal placental tissue. Three images per animal were captured and the percentage/number of blood vessels within the tissue area was analyzed. The data were analyzed using the GLM procedure of SAS. Results indicated that on d 83 COT vascularity was not affected by VTM (P-value = 0.5030), NRG (P-value = 0.5450), or their interaction (P-value = 0.6664), whereas on parturition there was a tendency for COT vascularity to be greater in VTM than CON (P- value=0.0723). These results suggest that dietary VTM supplementation from breeding until parturition potentially has a beneficial effect on placental vascular development in crossbred Angus heifers.