Enhancement of Radiosensitivity by CpG-Oligodeoxyribonucleotide-7909 in Human Non-Small Cell Lung Cancer A549 Cells
Enhancement of Radiosensitivity by CpG-Oligodeoxyribonucleotide-7909 in Human Non-Small Cell Lung Cancer A549 Cells
复制标题
DOI：
10.1089/cbr.2009.0686
复制
发表时间：
2010-04-01
影响因子：
3.4
通讯作者：
Lei, Linjie
中科院分区：
文献类型：
--
作者：
Zha, Lin;Qiao, Tiankui;Lei, Linjie
CpG-寡脱氧核糖核苷酸（CpG-ODN）通过Toll样受体9诱导信号传导，目前正被研究作为抗癌症的免疫刺激剂。最近有研究表明，CpG-ODNs还可以增强某些癌细胞系对传统疗法（包括化疗）的敏感性。本研究的目的是确定CpG-ODN 7909在体外增加人非小细胞肺癌细胞系A549的放射敏感性的活性。首先，在用不同浓度的CpG-ODN 7909（5、10、30和60 μ g/mL）处理A549细胞后，观察到对细胞活力的剂量和时间依赖性抑制作用。其次，与单独治疗相比，10 μ g/mL CpG-ODN 7909和放射联合治疗后发现细胞克隆形成存活率降低，细胞凋亡指数增加，G2/M期细胞百分比累积，肿瘤坏死因子（TNF-α）分泌增加（p
CpG-oligodeoxyribonucleotides (CpG-ODNs), which induce signaling through the toll-like receptor 9, are currently under investigation as immunity stimulators against cancer. It has recently been suggested that CpG-ODNs may also enhance sensitivity to traditional therapies including chemotherapy in certain cancer-cell lines. The purpose of this study was to define the activity of CpG-ODN7909 in increasing radiosensitivity of the human non-small cell lung cancer cell line A549 in vitro. First, a dose-and time-dependent inhibitory effect on cell viability was observed after A549 cells were treated with different concentrations of CpG-ODN7909 (5, 10, 30, and 60 mu g/mL). Second, decreased cell clonogenic survival, enhanced cell apoptotic index, accumulated percentage of cells in the G2/M phase, and increased tumor necrosis factor (TNF)-alpha secretion were found after combined treatments with 10 mu g/mL of CpG-ODN7909 and radiation compared to either treatment alone (p