Greenhouse Method for Studying Development and Survival of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)
Greenhouse Method for Studying Development and Survival of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)
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用于研究 Diabrotica virgifera virgifera（鞘翅目：叶甲科）发育和生存的温室方法
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发表时间：
1987
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通讯作者：
J. Fisher
中科院分区：
文献类型：
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作者：
J. Fisher
描述了一种具有适当验证的方法，用于定量研究单个或多个环境效应对西方玉米根萤叶甲（Diabrotica virgifera virgifera LeConte）、幼虫发育和存活至成虫阶段的影响。该方法使用受控环境温室（10 cm深的盆栽土壤温度为26 ± 5°C，相对湿度为45-65%，光周期为14：10 [L：D]）、赤陶盆（直径30.5 cm，高30.5 cm）和砂质粘壤土。土壤水分的调节与严格的浇水制度。通过每隔一天对花盆取样来确定初始发生率。这种情况与生长度日（GDD）有关，以11°C为基准，具有线性方程。方程的R2值为0.62，0.63，和0.69的第一，第二，和第三龄，分别预测50%的发生率为第一龄在74 GDD，第二龄在150.5 GDD，和第三龄在247.2 GDD。该方法还提供了相对均匀的成虫期存活率（67 ± 15% [ x ± SD]）。
A method with appropriate validation is described for quantitatively studying single or multiple environmental effects on western corn rootworm, Diabrotica virgifera virgifera LeConte, larva development, and survival to adult stage. The method used a controlled-environment greenhouse (26 ± 5°C pot soil temperature at 10 cm depth, 45–65% RH, 14:10 [L:D] photoperiod), terra-cotta clay pots (30.5 cm diameter by 30.5 cm high), and a sandy-clay loam soil. Soil moisture was regulated with a rigid watering regime. Instar occurrence was determined by sampling pots every other day. This occurrence was related to growing-degree-days (GDD), base 11°C, with linear equations. Equations with R2 values of 0.62, 0.63, and 0.69 for first, second, and third instars, respectively, predicted 50% occurrence for first instar at 74 GDD, second instar at 150.5 GDD, and third instar at 247.2 GDD. This method also provided a relatively uniform survival rate to the adult stage (67 ± 15% [ x ¯ ± SD]).