ELECTRON DETACHMENT DISSOCIATION AND INFRARED MULTIPHOTON DISSOCIATION OF HEPARIN TETRASACCHARIDES.
ELECTRON DETACHMENT DISSOCIATION AND INFRARED MULTIPHOTON DISSOCIATION OF HEPARIN TETRASACCHARIDES.
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DOI：
10.1016/j.ijms.2011.08.029
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发表时间：
2011-12-01
影响因子：
1.8
通讯作者：
Amster IJ
中科院分区：
文献类型：
--
作者：
Leach FE 3rd;Xiao Z;Laremore TN;Linhardt RJ;Amster IJ
由于高水平的硫酸化和结构异质性，肝素糖胺聚糖（GAG）是最难进行分析表征的糖型。最近临床肝素供应的污染和随后的死亡事件突出了对敏感分析方法的需求。在过去的十年中，串联质谱法已越来越多地应用于分析的糖胺酸，但在表征高度硫酸化的化合物的发展一直是最小的，由于碰撞诱导解离（CID）的阈值离子活化所产生的交叉环裂解的数量很少。本文采用电子解离（EDD）和红外多光子解离（IRMPD）技术对一系列肝素四糖进行了研究。用这两种活化方法，观察到丰富的糖苷和交叉环裂解。离子化硫酸盐标准（ISC）的概念是作为一个简洁的方法来描述的电荷状态，电离度和钠/质子交换的前体离子。这些因素有助于MS/MS测量期间有用碎片的倾向。ISC值为0的前体在这里进行了研究，并显示出足够的结构信息，从离子活化EDD或IRMPD。
Heparin glycosaminoglycans (GAGs) present the most difficult glycoform for analytical characterization due to high levels of sulfation and structural heterogeneity. Recent contamination of the clinical heparin supply and subsequent fatalities has highlighted the need for sensitive methodologies of analysis. In the last decade, tandem mass spectrometry has been increasingly applied for the analysis of GAGs, but developments in the characterization of highly sulfated compounds have been minimal due to the low number of cross-ring cleavages generated by threshold ion activation by collisional induced dissociation (CID). In the current work, electron detachment dissociation (EDD) and infrared multiphoton dissociation (IRMPD) are applied to a series of heparin tetrasaccharides. With both activation methods, abundant glycosidic and cross-ring cleavages are observed. The concept of Ionized Sulfate Criteria (ISC) is presented as a succinct method for describing the charge state, degree of ionization and sodium/proton exchange in the precursor ion. These factors contribute to the propensity for useful fragmentation during MS/MS measurements. Precursors with ISC values of 0 are studied here, and shown to yield adequate structural information from ion activation by EDD or IRMPD.
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影响因子：
7.4
作者：
Saad, OM;Leary, JA
通讯作者：
Leary, JA
影响因子：
46.9
作者：
Guerrini, Marco;Beccati, Daniela;Sasisekharan, Ram
通讯作者：
Sasisekharan, Ram
影响因子：
2.9
作者：
GUO, Y;CONRAD, HE
通讯作者：
CONRAD, HE
DOI：
10.1016/j.jasms.2007.10.007
发表时间：
2008-02-01
影响因子：
3.2
作者：
Wolff, Jeremy J.;Laremore, Tatiana N.;Amster, I. Jonathan
通讯作者：
Amster, I. Jonathan
影响因子：
3.1
作者：
JUHASZ, P;BIEMANN, K
通讯作者：
BIEMANN, K