Aquaglyceroporins Are Differentially Expressed in Beige and White Adipocytes
Aquaglyceroporins Are Differentially Expressed in Beige and White Adipocytes
复制标题
水甘油通道蛋白在米色和白色脂肪细胞中的差异表达
DOI：
10.3390/ijms21020610
复制
发表时间：
2020-01-02
影响因子：
5.6
通讯作者：
Soveral, Graca
中科院分区：
文献类型：
--
作者：
da Silva, Ines Vieira;Diaz-Saez, Francisco;Soveral, Graca
白色脂肪细胞的布朗宁已被提出作为克服代谢并发症的有力策略，因为棕色脂肪细胞更分解代谢，以热形式消耗能量。然而，参与布朗宁过程的生物学途径仍然不清楚。水甘油通道蛋白是水通道蛋白水通道的一个亚类，其也渗透甘油并参与身体能量稳态。在脂肪组织中，水通道蛋白7（AQP 7）是最具代表性的亚型，对白色脂肪细胞的完全分化和甘油代谢至关重要。AQP 7表达的改变与肥胖和代谢紊乱的发生有关。在此，我们研究了水甘油孔蛋白是否与米色脂肪细胞分化有关，类似于白色细胞。因此，我们优化了小鼠3 T3-L1前脂肪细胞布朗宁的方案，该方案在基因和蛋白质水平上显示出增加的米色和减少的白色脂肪组织特征，并评估了水通道蛋白表达模式沿着分化过程以及细胞脂质含量。我们的研究结果表明，AQP 7和水通道蛋白-9（AQP 9）的表达在整个米色脂肪细胞分化下调相比，白色分化，这可能与米色的生理作用，从氧化代谢产生的热量，与合成代谢/分解代谢脂质代谢需要甘油网关发生在白色脂肪细胞。
Browning of white adipocytes has been proposed as a powerful strategy to overcome metabolic complications, since brown adipocytes are more catabolic, expending energy as a heat form. However, the biological pathways involved in the browning process are still unclear. Aquaglyceroporins are a sub-class of aquaporin water channels that also permeate glycerol and are involved in body energy homeostasis. In the adipose tissue, aquaporin-7 (AQP7) is the most representative isoform, being crucial for white adipocyte fully differentiation and glycerol metabolism. The altered expression of AQP7 is involved in the onset of obesity and metabolic disorders. Herein, we investigated if aquaglyceroporins are implicated in beige adipocyte differentiation, similar to white cells. Thus, we optimized a protocol of murine 3T3-L1 preadipocytes browning that displayed increased beige and decreased white adipose tissue features at both gene and protein levels and evaluated aquaporin expression patterns along the differentiation process together with cellular lipid content. Our results revealed that AQP7 and aquaporin-9 (AQP9) expression was downregulated throughout beige adipocyte differentiation compared to white differentiation, which may be related to the beige physiological role of heat production from oxidative metabolism, contrasting with the anabolic/catabolic lipid metabolism requiring glycerol gateways occurring in white adipose cells.