Functionalized BaTiO3 enhances piezoelectric effect towards cell response of bone scaffold
Functionalized BaTiO3 enhances piezoelectric effect towards cell response of bone scaffold
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功能化 BaTiO3 增强骨支架细胞反应的压电效应
DOI：
10.1016/j.colsurfb.2019.110587
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发表时间：
2020-01-01
影响因子：
5.8
通讯作者：
Peng, Shuping
中科院分区：
文献类型：
--
作者：
Shuai, Cijun;Liu, Guofeng;Peng, Shuping
聚偏氟乙烯（PVDF）的压电效应对骨组织内源性电微环境的恢复起着至关重要的作用，而PVDF中β相含量越多，压电性能越好。纳米颗粒可以诱导β相的成核。但它们在PVDF基体中易发生聚集，导致β相的成核能力减弱。在这项工作中，羟基化的钛酸钡纳米粒子与聚多巴胺功能化，以促进其在PVDF支架通过选择性激光烧结制造的分散。一方面，聚多巴胺的邻苯二酚基团可以与钛酸钡的羟基形成氢键。另一方面，聚多巴胺的氨基能够与PVDF的C-F基团键合。结果表明，功能化的BaTiO 3纳米粒子均匀分布在PVDF基体中，使β相含量从46%提高到59%，输出电压提高了356%。细胞测试证实增强的表面电刺激显著促进细胞粘附、增殖和分化。此外，支架表现出增强的拉伸强度和模量，这归因于刚性颗粒增强效应和改善的界面粘附。该研究表明，压电支架在骨修复中显示出潜在的应用前景。
Piezoelectric effect of polyvinylidene fluoride (PVDF) plays a crucial role in restoring the endogenous electrical microenvironment of bone tissue, whereas more beta phase in PVDF leads to higher piezoelectric performance. Nanoparticles can induce the nucleation of the beta phase. However, they are prone to aggregate in PVDF matrix, resulting in weakened nucleation ability of beta phase. In this work, the hydroxylated BaTiO3 nanoparticles were functionalized with polydopamine to promote their dispersion in PVDF scaffolds fabricated via selective laser sintering. On one hand, the catechol groups of polydopamine could form hydrogen bonding with the hydroxyl groups of the BaTiO3. On the other hand, the amino groups of polydopamine were able to bond with C-F group of PVDF. As a result, the functionalized BaTiO3 nanoparticles homogeneously distributed in PVDF matrix, which significantly increased the beta phase fraction from 46% to 59% with an enhanced output voltage by 356%. Cell testing confirmed the enhanced surface electric cues significantly promoted cell adhesion, proliferation and differentiation. Furthermore, the scaffolds exhibited enhanced tensile strength and modulus, which was ascribed to the rigid particle strengthening effect and the improved interfacial adhesion. This study suggested that the piezoelectric scaffolds shown a potential application in bone repair.