Tumor-associated transplantation antigens in immune rejection of mouse malignant cell hybrids.
Tumor-associated transplantation antigens in immune rejection of mouse malignant cell hybrids.
复制标题
小鼠恶性细胞杂种免疫排斥中的肿瘤相关移植抗原。
DOI：
10.1073/pnas.72.6.2130
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发表时间：
1975
影响因子：
11.1
通讯作者：
E. Ritz
中科院分区：
文献类型：
--
作者：
J. Jami;E. Ritz
分析了来自两个恶性亲本细胞的小鼠细胞杂交体在同基因F1宿主中不能产生肿瘤。来自C1.1D和L1210 clls融合的YC杂交体未能在成年小鼠中诱导任何肿瘤，而91%的X射线照射的新生小鼠发生肿瘤并死亡。一些端着丝粒染色体缺乏杂交肿瘤，然而，没有免疫完整的成年小鼠肿瘤移植肿瘤生长在x射线照射的新生小鼠。这表明组织相容性因素干扰了YC肿瘤生长至成年宿主的失败。用Y2 C杂交细胞免疫的同系F1小鼠具有针对Y2 C杂交细胞以及C1.1D和L1210亲代细胞的细胞毒性抗体。两种亲本细胞系的混合物对杂交细胞的细胞毒性抗体的完全吸收表明杂交细胞上不存在任何新的抗原。这可能解释了杂交细胞的较高抗原性和/或对免疫裂解的较高敏感性。
The inability of mouse cell hybrids derived from two malignant parental cells to produce tumors in syngeneic F1 hosts was analyzed. YC hybrids, derived from the fusion of C1.1D and L1210 clls, failed to induce any tumor in adult mice, while 91% of x-irradiated newborn mice developed tumors and died. Some telocentric chromosomes were lacking in hybrid tumors; however, none of the immunologically intact adult mice developed tumors when grafted with tumors grown in x-irradiated newborn mice. This indicates that histocompatibility factors interfered in the failure of YC tumors to grow to adult hosts. Syngeneic F1 mice immunized with Y2C hybrid cells had cytotoxic antibodies against Y2C hybrids and C1.1D and L1210 parental cells. Complete absorption of cytotoxic antibodies directed against hybrid cells by mixtures of both parental cell lines demonstrates the absence of any new antigen on hybrid cells.Hyb rid cells had a higher density of C1.1D-tumor antigenic sites, as compared to C1.1D parental cells. This possibly explains the higher antigenicity and/or the higher sensitivity to immune lysis of hybrid cells.