TILLING. Traditional mutagenesis meets functional genomics
TILLING. Traditional mutagenesis meets functional genomics
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DOI：
10.1104/pp.104.041061
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发表时间：
2004-06-01
期刊：
影响因子：
7.4
通讯作者：
Comai, L
中科院分区：
文献类型：
--
作者：
Henikoff, S;Till, BJ;Comai, L
生物体的大多数基因都是已知的序列，但大多数表型是模糊的。因此，反向遗传学已成为许多生物学家的重要目标。然而，反向遗传学方法并不同样适用于所有生物。在反向遗传学的一般策略中，我们称之为TILLING（基因组中的靶向诱导局部病变），传统的化学诱变之后是点突变的高通量筛选。TILLING承诺普遍适用。此外，由于TILLING不涉及转基因修饰，因此它不仅对功能基因组学而且对农业应用都有吸引力。在这里，我们提出了TILLING方法，包括生态耕作，这需要检测自然变异的状态的概述。我们描述了公开的TILLING努力在拟南芥和其他生物，包括玉米（玉米）和斑马鱼。我们的结论是TILLING，在植物中开发的技术，正在迅速被其他系统采用。
Most of the genes of an organism are known from sequence, but most of the phenotypes are obscure. Thus, reverse genetics has become an important goal for many biologists. However, reverse-genetic methodologies are not similarly applicable to all organisms. In the general strategy for reverse genetics that we call TILLING (for Targeting Induced Local Lesions in Genomes), traditional chemical mutagenesis is followed by high-throughput screening for point mutations. TILLING promises to be generally applicable. Furthermore, because TILLING does not involve transgenic modifications, it is attractive not only for functional genomics but also for agricultural applications. Here, we present an overview of the status of TILLING methodology, including Ecotilling, which entails detection of natural variation. We describe public TILLING efforts in Arabidopsis and other organisms, including maize (Zea mays) and zebrafish. We conclude that TILLING, a technology developed in plants, is rapidly being adopted in other systems.