Estrogen synthesis in relation to gonadal development of Japanese scallop, Patinopecten yessoensis:: gonadal profile and immunolocalization of P450 aromatase and estrogen
Estrogen synthesis in relation to gonadal development of Japanese scallop, Patinopecten yessoensis:: gonadal profile and immunolocalization of P450 aromatase and estrogen
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DOI：
10.1016/j.cbpc.2004.07.002
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发表时间：
2004-09-01
影响因子：
2.2
通讯作者：
Mori, K
中科院分区：
文献类型：
--
作者：
Osada, M;Tawarayama, H;Mori, K
采用免疫组织化学方法测定了日本扇贝（Patinopecten yessoensis）性腺中芳香酶活性和雌激素含量，并鉴定了睾丸中的雌激素细胞。1 - 2月卵巢和睾丸发育迅速，3月成熟，4月产卵。卵巢和睾丸芳香化酶活性的增加早于卵巢和睾丸发育的开始。芳香酶活性在2月生长期和3月成熟期达到最高水平，在4月产卵期显著下降。卵巢和睾丸中雌二醇-17 β含量的变化与卵巢和睾丸中芳香化酶活性的变化相似，但雌酮没有变化。在睾丸腺泡壁内侧的细胞中检测到对P450芳香化酶和雌二醇-17 β的免疫反应，而在以前的报道中，细胞分布在卵巢腺泡壁外侧。本研究提示雌激素是通过P450芳香化酶在卵巢和睾丸的雌激素细胞中合成的，睾丸雌激素可能在精子发生中发挥生理作用。(C) 2004爱思唯尔公司版权所有。
Aromatase activities and estrogen contents in the gonad of Japanese scallop, Patinopecten yessoensis, were determined during gonadal development and estrogenic cells in the testis were identified immunohistochemically. Ovaries and testes developed rapidly during January and February to reach the mature stage in March and the spawning stage in April. Increases in aromatase activities of the ovary and testis preceded the onset of the ovarian and testicular development. Aromatase activities reached the highest level at the growing stage in February and the mature stage in March, and showed a striking decrease at the spawning stage in April. Contents of ovarian and testicular estradiol-17beta changed similarly to the profile of aromatase activities in the ovary and testis, although estrone showed no change. Immunoreactivities against P450 aromatase and estradiol-17beta were detected in the cells along the inside of the acinar wall of the testis, whereas in the previous reports, the cells are distributed along the outside of the acinar wall in the ovary. This study thus suggests that estrogen is synthesized in the estrogenic cells of the ovary and testis through aromatization by P450 aromatase and that testicular estrogen may play a physiological role in spermatogenesis. (C) 2004 Elsevier Inc. All rights reserved.