Trefoil factors are expressed in human and rat endocrine pancreas:: Differential regulation by growth hormone
Trefoil factors are expressed in human and rat endocrine pancreas:: Differential regulation by growth hormone
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DOI：
10.1210/en.2006-0601
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发表时间：
2006-12-01
期刊：
影响因子：
4.8
通讯作者：
Nielsen, Jens H.
中科院分区：
文献类型：
--
作者：
Jackerott, Malene;Lee, Ying C.;Nielsen, Jens H.
三叶因子（TFFs）1、2和3在粘膜上皮中表达。TFF在肠中特别丰富，其中它们在上皮的维持和恢复中起关键作用。由于胰腺发育起源于原始前肠，我们探索了TFF在人和大鼠胰腺中的表达。免疫细胞化学染色显示，在成人胰腺的胰岛和一些导管细胞丰富的TFF 3免疫反应，而弱的TFF 1和没有TFF 2染色检测。在胰岛中，TFF 3定位于大多数胰岛素和一些胰高血糖素和胰多肽产生细胞。TFF 3免疫反应共定位与胰岛素和胰高血糖素在不同的细胞群在人胎儿胰腺在第14周和新生大鼠胰腺。在分离的人和大鼠胰岛中，通过RT-PCR鉴定TFF 3和TFF 1 mRNA，并通过ELISA检测人胰腺和胰岛中的TFF 3蛋白。暴露于GH（一种已知的β细胞生长因子）的新生大鼠胰岛或胰岛素瘤细胞导致TFF 3显著增加，但TFF 1 mRNA水平降低。通过Western blot证实GH对TFF 3表达的影响。在TFF 3存在下培养新生大鼠胰岛导致胰岛细胞的附着和迁移，但对增殖、胰岛素分泌或精氨酸诱导的凋亡没有影响。这些数据证明了TFF在内分泌胰腺中的表达，但其可能的功能仍然未知。
Trefoil factors (TFFs) 1, 2, and 3 are expressed in mucosal epithelia. TFFs are particular abundant in the intestine in which they play a crucial role in maintenance and restitution of the epithelium. Because pancreas developmentally arises from the primitive foregut, we explored the expression of TFFs in the pancreas in man and rat. Immunocytochemical staining of adult human pancreas showed abundant TFF3 immunoreactivity in pancreatic islets and some duct cells, whereas weak TFF1 and no TFF2 staining were detected. In the islets TFF3 localized to most insulin and some glucagon and pancreatic polypeptide-producing cells. TFF3 immunoreactivity was colocalized with insulin and glucagon in distinct cell clusters in human fetal pancreas at wk 14 and in the newborn rat pancreas. In isolated human and rat islets, TFF3 and TFF1 mRNA was identified by RT-PCR, and TFF3 protein was detected in human pancreas and islets by ELISA. Exposure of neonatal rat islets or insulinoma cells to GH, a known beta-cell growth factor, resulted in markedly increased TFF3 but decreased TFF1 mRNA levels. The effect of GH on TFF3 expression was confirmed by Western blot. Culture of neonatal rat islets in the presence of TFF3 resulted in attachment and migration of the islet cells, but no effects on proliferation, insulin secretion or cytokine-induced apoptosis were seen. These data demonstrate expression of TFFs in the endocrine pancreas, but their possible functions remain unknown.