Genetic diversity of the Yokose virus, XYBX1332, isolated from bats (Myotis daubentonii) in China
Genetic diversity of the Yokose virus, XYBX1332, isolated from bats (Myotis daubentonii) in China
复制标题
从中国蝙蝠 (Myotis daubentonii) 中分离出的 Yokose 病毒 XYBX1332 的遗传多样性
DOI：
10.1186/s12985-018-1107-3
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发表时间：
2019-01-11
期刊：
影响因子：
4.8
通讯作者：
Liang, Guodong
中科院分区：
文献类型：
--
作者：
Feng, Yun;Ren, Xiaojie;Liang, Guodong
Yokose病毒是1971年从日本横须贺地区采集的蝙蝠(Miniopterus Fuliginosus)上首次分离到的，是黄病毒属黄病毒科黄病毒属的新成员。本研究于2013年从云南省中国采集的蝙蝠血清标本中分离到一株Yokose病毒。方法用蝙蝠血清标本接种BHK-21和Vero E6细胞进行病毒分离。结果该病毒在哺乳动物细胞(BHK-21和Vero E6)中具有细胞病变效应。基因组测序表明，它有一个单一的开放阅读框架(ORF)，基因组总长度为10,785个核苷酸。病毒基因组的系统发育分析表明，XYBX1332是黄病毒属的一种YOKV。YOKV原始毒株XYBX1332和OITA-36的ORF核苷酸同源性为72%，氨基酸同源性为82%。XYBX1332和OITA-36的ORF分别编码3422和3425个氨基酸。此外，这两个毒株的非编码区(5-和3-非编码区)的长度也不同，5-和3-UTRs的同源性分别为81.5%和78.3%。结论YOKV(XYBX1332)从距离日本横须贺数千公里的内陆中国分离，表明YOKV的地理分布不仅限于日本岛屿，它还可以存在于亚洲内陆地区。然而，我国YOKV毒株与日本YOKV毒株在基因组上存在较大差异。
BackgroundYokose virus was first isolated from bats (Miniopterus fuliginosus) collected in Yokosuka, Japan, in 1971, and is a new member of the family Flaviviridae, genus Flavivirus. In this study, we isolated a Yokose virus from a serum sample of Myotis daubentonii (order Chiroptera, family Vespertilionidae) collected in Yunnan province, China in 2013.MethodsThe serum specimens of bat were used to inoculate in BHK-21 and Vero E6 cells for virus isolation. Then the viral complete genome sequence was obtained and was used for phylogenetic analysis performed by BEAST software package.ResultsThe virus was shown to have cytopathic effects in mammalian cells (BHK-21 and Vero E6). Genome sequencing indicated that it has a single open reading frame (ORF), with a genome of 10,785 nucleotides in total. Phylogenetic analysis of the viral genome suggests that XYBX1332 is a Yokose virus (YOKV) of the genus Flavivirus. Nucleotide and amino acid homology levels of the ORF of XYBX1332 and Oita-36, the original strain of YOKV, were 72 and 82%, respectively. The ORFs of XYBX1332 and Oita-36 encode 3422 and 3425 amino acids, respectively. In addition, the non-coding regions (5- and 3-untranslated regions [UTRs]) of these two strains differ in length and the homology of the 5- and 3-UTRs was 81.5 and 78.3%, respectively.ConclusionThe isolation of YOKV (XYBX1332) from inland China thousands of kilometers from Yokosuka, Japan, suggests that the geographical distribution of YOKV is not limited to the islands of Japan and that it can also exist in the inland areas of Asia. However, there are large differences between the Chinese and Japanese YOKV strains in viral genome.