Differences in Stylet Sheath Occurrence and the Fibrous Ring (Sclerenchyma) between xCitroncirus Plants Relatively Resistant or Susceptible to Adults of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Liviidae)
Differences in Stylet Sheath Occurrence and the Fibrous Ring (Sclerenchyma) between xCitroncirus Plants Relatively Resistant or Susceptible to Adults of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Liviidae)
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DOI：
10.1371/journal.pone.0110919
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发表时间：
2014-10-24
期刊：
影响因子：
3.7
通讯作者：
Shatters, Robert G., Jr.
中科院分区：
文献类型：
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作者：
Ammar, El-Desouky;Richardson, Matthew L.;Shatters, Robert G., Jr.
亚洲柑橘木虱（ACP，Diaphorina citri，半翅目：Liviidae）是与黄龙病（HLB）密切相关的韧皮部细菌的主要媒介，黄龙病是世界上最严重的柑橘病害。宿主植物抗性可以提供一种环境安全且可持续的控制 ACP 和/或 HLB 的方法。就成虫寿命而言，对 ACP 成虫相对抗性 (UN-3881) 或相对敏感 (Troyer-1459) 的两个 xCitroncirus 种质（枳属和柑橘属的杂交品种）的 ACP 取食行为和叶中脉的一些结构特征进行了比较。 ACP 成虫在叶子不同部分的定居（假定的取食/探查）位点主要不受植物加入的影响。然而，与 Troyer-1459 植物相比，UN-3881 中的中脉中发现的 ACP 管心鞘较少，到达维管束（韧皮部和/或木质部）的管芯鞘末端也较少。此外，与 Troyer-1459 叶子的中脉相比，UN-3881 叶子的中脉中韧皮部周围的纤维环（厚壁组织）明显更宽（更厚）。我们的数据表明，与更敏感的品种 (Troyer-1459) 相比，抵抗力更强的 (UN-3881) 成虫对维管束的进食和/或探查频率较低。我们的结果还表明，纤维环的厚度可能是管心针穿透维管束的障碍，这对于 ACP 成功进食韧皮部和传播 HLB 相关细菌非常重要。这些结果可能有助于培育对 ACP 具有抗性的柑橘植物，从而阻止或减缓该载体引起的 HLB 相关细菌的传播。
The Asian citrus psyllid (ACP, Diaphorina citri, Hemiptera: Liviidae), is the principal vector of the phloem-limited bacteria strongly associated with huanglongbing (HLB), the world's most serious disease of citrus. Host plant resistance may provide an environmentally safe and sustainable method of controlling ACP and/or HLB. Two xCitroncirus accessions (hybrids of Poncirus trifoliata and Citrus spp.), that are relatively resistant (UN-3881) or relatively susceptible (Troyer-1459) to ACP adults with regard to adult longevity, were compared in relation to ACP feeding behavior and some structural features of the leaf midrib. The settling (putative feeding/probing) sites of ACP adults on various parts of the leaf were not influenced primarily by plant accession. However, fewer ACP stylet sheaths were found in the midrib and fewer stylet sheath termini reached the vascular bundle (phloem and/or xylem) in UN-3881 compared to Troyer-1459 plants. Furthermore, in midribs of UN-3881 leaves the fibrous ring (sclerenchyma) around the phloem was significantly wider (thicker) compared to that in midribs of Troyer-1459 leaves. Our data indicate that feeding and/or probing by ACP adults into the vascular bundle is less frequent in the more resistant (UN-3881) than in the more susceptible (Troyer-1459) accessions. Our results also suggest that the thickness of the fibrous ring may be a barrier to stylet penetration into the vascular bundle, which is important for successful ACP feeding on the phloem and for transmitting HLB-associated bacteria. These results may help in the development of citrus plants resistant to ACP, which in turn could halt or slow the spread of the HLB-associated bacteria by this vector.