Stem-cell-based tissue engineering of murine teeth
Stem-cell-based tissue engineering of murine teeth
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DOI：
10.1177/154405910408300702
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发表时间：
2004-07-01
影响因子：
7.6
通讯作者：
Sharpe, PT
中科院分区：
文献类型：
--
作者：
Ohazama, A;Modino, SAC;Sharpe, PT
牙齿是由间充质细胞和上皮相互作用形成的，其中上皮为牙齿形成提供了指导性信息。基于这些最初的组织相互作用，我们在小鼠体内用培养的非牙干细胞聚集产生的间充质细胞代替了间充质细胞。非牙细胞来源的间充质和胚胎口腔上皮之间的重组刺激干细胞的成牙反应。胚胎干细胞、神经干细胞和成体骨髓来源的细胞都通过表达成牙基因做出反应。将重组体转移到成人肾囊中，导致牙齿结构和相关骨的发育。此外，胚胎牙原基移植到成年颌骨后，牙齿结构得以发育，这表明胚胎牙原基可以在其成年环境中发育。因此，这些结果为从培养细胞中创造人工胚胎牙原基提供了重要的进展，这些细胞可以在移植到成人口腔后用于替代缺失的牙齿。
Teeth develop from reciprocal interactions between mesenchyme cells and epithelium, where the epithelium provides the instructive information for initiation. Based on these initial tissue interactions, we have replaced the mesenchyme cells with mesenchyme created by aggregation of cultured non-dental stem cells in mice. Recombinations between non-dental cell-derived mesenchyme and embryonic oral epithelium stimulate an odontogenic response in the stem cells. Embryonic stem cells, neural stem cells, and adult bone-marrow-derived cells all responded by expressing odontogenic genes. Transfer of recombinations into adult renal capsules resulted in the development of tooth structures and associated bone. Moreover, transfer of embryonic tooth primordia into the adult jaw resulted in development of tooth structures, showing that an embryonic primordium can develop in its adult environment. These results thus provide a significant advance toward the creation of artificial embryonic tooth primordia from cultured cells that can be used to replace missing teeth following transplantation into the adult mouth.